Division 4 The reagents of the tests shall consist of:F1F2

antigen: the antigen shall contain specific glycoprotein of enzootic bovine leukosis virus which has been standardised against the E05 serum;

the test serum;

known positive control serum;

agar gel:

• 0,8 % agar,

• 8,5 % NaCl,

• 0,05 M Tris-buffer pH 7,2,

• 15 ml of this agar shall be introduced into a petri dish of 85 mm diameter, resulting in a depth of 2,6 mm of agar.