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Third Commission Directive of 27 April 1972 establishing Community methods of analysis for the official control of feedingstuffs (72/199/EEC) (repealed)

EU Directive 1972/199 · Adopted 27 April 1972 · Extends to United Kingdom

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Third Commission Directive

of 27 April 1972

establishing Community methods of analysis for the official control of feedingstuffs

(72/199/EEC) (repealed)

F1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Annotations to the whole text

Textual amendments

F1

Repealed by Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance).

Contents

  1. 1Article 1 Repealed
  2. 2Article 2 Repealed
  3. 3Article 3 Repealed
  4. 4Article 4 Repealed
  5. Annex I Repealed
  6. 1DETERMINATION OF STARCH Repealed
  7. 1Purpose and scope Repealed
  8. 2Principle Repealed
  9. Reagents Repealed
  10. 3.1Division 3.1 Repealed
  11. 3.2Division 3.2 Repealed
  12. 3.3Division 3.3 Repealed
  13. 3.4Division 3.4 Repealed
  14. 3.5Division 3.5 Repealed
  15. Apparatus Repealed
  16. 4.1Division 4.1 Repealed
  17. 4.2Division 4.2 Repealed
  18. Procedure Repealed
  19. 5.1Preparation of the sample Repealed
  20. 5.2Determination of the total optical rotation (P or S) (see observation 7.1) Repealed
  21. 5.3Determination of the optical rotation (P' or S') of substances soluble in 40 % ethanol Repealed
  22. 6Calculation of results Repealed
  23. 6.1Measurement by polarimeter Repealed
  24. 6.2Measurement by saccharimeter Repealed
  25. 6.3Repeatability Repealed
  26. Observations Repealed
  27. 7.1Division 7.1 Repealed
  28. 7.2Division 7.2 Repealed
  29. 7.3Following feed materials, in case they are present in significant amount in feedingstuffs, are known to give rise to interferences when determining the starch content by polarimetric method and whereby incorrect results could be yielded: Repealed
  30. DETERMINATION OF CRUDE PROTEIN Repealed
  31. 1Purpose and scope Repealed
  32. 2Principle Repealed
  33. Reagents Repealed
  34. 3.1Division 3.1 Repealed
  35. 3.2Division 3.2 Repealed
  36. 3.3Division 3.3 Repealed
  37. 3.4Division 3.4 Repealed
  38. 3.5Division 3.5 Repealed
  39. 3.6Division 3.6 Repealed
  40. 3.7Division 3.7 Repealed
  41. 3.8Division 3.8 Repealed
  42. 3.9Division 3.9 Repealed
  43. 3.10Division 3.10 Repealed
  44. 3.11Division 3.11 Repealed
  45. 3.12Division 3.12 Repealed
  46. 3.13Division 3.13 Repealed
  47. 4Apparatus Repealed
  48. Procedure Repealed
  49. 5.1Digestion Repealed
  50. 5.2Distillation Repealed
  51. 5.3Titration Repealed
  52. 5.4Blank test Repealed
  53. 6Calculation of results Repealed
  54. Verification of the method Repealed
  55. 7.1Repeatability Repealed
  56. 7.2Accuracy Repealed
  57. Observations Repealed
  58. 8.1Division 8.1 Repealed
  59. 8.2Division 8.2 Repealed
  60. 8.3Division 8.3 Repealed
  61. DETERMINATION OF CRUDE PROTEIN DISSOLVED BY PEPSIN AND HYDROCHLORIC ACID Repealed
  62. 1Purpose and scope Repealed
  63. 2Principle Repealed
  64. Reagents Repealed
  65. 3.1Division 3.1 Repealed
  66. 3.2Division 3.2 Repealed
  67. 3.3Division 3.3 Repealed
  68. 3.4Division 3.4 Repealed
  69. 3.5Division 3.5 Repealed
  70. 3.6Division 3.6 Repealed
  71. Apparatus Repealed
  72. 4.1Division 4.1 Repealed
  73. 4.2Division 4.2 Repealed
  74. Procedure Repealed
  75. 5.1Preparation of solution (see observation 7.2) Repealed
  76. 5.2Digestion Repealed
  77. 5.3Distillation and titration Repealed
  78. 5.4Blank test Repealed
  79. 6Calculation of results Repealed
  80. Repeatability Repealed
  81. Observations Repealed
  82. 7.1Division 7.1 Repealed
  83. 7.2Division 7.2 Repealed
  84. ESTIMATION OF PEPSIN ACTIVITY Repealed
  85. 1Purpose and scope Repealed
  86. 2Principle Repealed
  87. Reagents Repealed
  88. 3.1Division 3.1 Repealed
  89. 3.2Division 3.2 Repealed
  90. 3.3Division 3.3 Repealed
  91. 3.4Division 3.4 Repealed
  92. 3.5Division 3.5 Repealed
  93. 3.6Division 3.6 Repealed
  94. 3.7Division 3.7 Repealed
  95. 3.8Division 3.8 Repealed
  96. Apparatus Repealed
  97. 4.1Division 4.1 Repealed
  98. 4.2Division 4.2 Repealed
  99. 4.3Division 4.3 Repealed
  100. 4.4Division 4.4 Repealed
  101. Procedure Repealed
  102. 5.1Preparation of the solution (see observation 7.1) Repealed
  103. 5.2Hydrolysis Repealed
  104. 5.3Development of coloration and measurement of optical density Repealed
  105. 5.4Blank test Repealed
  106. 5.5Calibration curve Repealed
  107. 6Calculation of results Repealed
  108. Observations Repealed
  109. 7.1Division 7.1 Repealed
  110. 7.2Division 7.2 Repealed
  111. DETERMINATION OF FREE AND GOSSYPOL Repealed
  112. 1Purpose and scope Repealed
  113. 2Principle Repealed
  114. 3Reagents Repealed
  115. 3.1Division 3.1 Repealed
  116. 3.2Division 3.2 Repealed
  117. 3.3Division 3.3 Repealed
  118. 3.4Division 3.4 Repealed
  119. 3.5Division 3.5 Repealed
  120. 3.6Division 3.6 Repealed
  121. Apparatus Repealed
  122. 4.1Division 4.1 Repealed
  123. 4.2Division 4.2 Repealed
  124. Procedure Repealed
  125. 5.1Test sample Repealed
  126. 5.2Determination of free gossypol Repealed
  127. 5.3Determination of total gossypol Repealed
  128. 6Calculation of results Repealed
  129. 6.1From the specific optical density Repealed
  130. From a calibration curve Repealed
  131. 6.2.1Free gossypol Repealed
  132. 6.2.2Total gossypol Repealed
  133. 6.3Repeatability Repealed
  134. Annex II Repealed
  135. DETECTION AND IDENTIFICATION OF ANTIBIOTICS OF THE TETRACYCLINE GROUP Repealed
  136. 1Purpose and scope Repealed
  137. 2Principle Repealed
  138. Reagents and culture medium Repealed
  139. 3.1Buffer solution, pH 3.5 Repealed
  140. 3.2Phosphate buffer solution, pH 5.5 Repealed
  141. 3.3Division 3.3 Repealed
  142. 3.4Division 3.4 Repealed
  143. 3.5Division 3.5 Repealed
  144. 3.6Division 3.6 Repealed
  145. 3.7Division 3.7 Repealed
  146. 3.8Division 3.8 Repealed
  147. 3.9Micro-organism: B. cereus ATCC No 11.778 Repealed
  148. 3.10Culture medium Repealed
  149. 3.11Division 3.11 Repealed
  150. Apparatus Repealed
  151. 4.1Division 4.1 Repealed
  152. 4.2Division 4.2 Repealed
  153. 4.3Division 4.3 Repealed
  154. 4.4Division 4.4 Repealed
  155. 4.5Division 4.5 Repealed
  156. Standard solutions Repealed
  157. 5.1Stock solutions Repealed
  158. 5.2Reference solutions for detections by UV light Repealed
  159. 5.3Reference solutions for detection by bioautography Repealed
  160. 6Extraction Repealed
  161. Detection and identification Repealed
  162. 7.1Chromatography Repealed
  163. 7.2Detection by UV light Repealed
  164. 7.3Detection by bioautography Repealed
  165. 7.4Identification Repealed
  166. DETERMINATION OF CHLORTETRACYCLINE, OXYTETRACYCLINE AND TETRACYCLINE Repealed
  167. BY DIFFUSION ON AGAR Repealed
  168. 1Purpose and scope Repealed
  169. 2Principle Repealed
  170. Micro-organism: B. cereus, ATCC No 11.778 Repealed
  171. 3.1Maintenance of the parent strain Repealed
  172. 3.2Preparation of the spore suspension Repealed
  173. Culture media and reagents Repealed
  174. 4.1Basic medium for the determination Repealed
  175. 4.2Phosphate buffer solution, pH 5·5 Repealed
  176. 4.3Division 4.3 Repealed
  177. 4.4Phosphate buffer solution, pH 8 Repealed
  178. 4.5Division 4.5 Repealed
  179. 4.6Division 4.6 Repealed
  180. 4.7Division 4.7 Repealed
  181. 4.8Division 4.8 Repealed
  182. 4.9Division 4.9 Repealed
  183. 4.10Division 4.10 Repealed
  184. 4.11Division 4.11 Repealed
  185. Standard solutions Repealed
  186. 5.1Chlortetracycline Repealed
  187. 5.2Oxytetracycline Repealed
  188. 5.3Tetracycline Repealed
  189. Extraction Repealed
  190. 6.1Contents of 50 ppm or less Repealed
  191. Contents greater than 50 ppm Repealed
  192. 6.2.1Chlortetracycline Repealed
  193. 6.2.2Oxytetracycline and tetracycline Repealed
  194. Determination method Repealed
  195. 7.1Inoculation of the culture medium Repealed
  196. 7.2Preparation of the trays Repealed
  197. 7.3Incubation Repealed
  198. 8Evaluation Repealed
  199. 9Repeatability Repealed
  200. BY TURBIDIMETRY Repealed
  201. 1Purpose and scope Repealed
  202. 2Principle Repealed
  203. Micro-organism: Staphylococcus aureus K 141 Repealed
  204. 3.1Maintenance of the parent strain Repealed
  205. 3.2Preparation of the inoculum Repealed
  206. Culture media and reagents Repealed
  207. 4.1Basic medium for the determination Repealed
  208. 4.2Phosphate buffer solution, pH 4·5 Repealed
  209. 4.3Division 4.3 Repealed
  210. 4.4Division 4.4 Repealed
  211. 4.5Division 4.5 Repealed
  212. 4.6Division 4.6 Repealed
  213. 4.7Division 4.7 Repealed
  214. 5Standard solution Repealed
  215. Extraction Repealed
  216. 6.1Chlortetracycline Repealed
  217. 6.2Oxytetracycline and tetracycline Repealed
  218. Determination method Repealed
  219. 7.1Preparation of the standard series and of the extract Repealed
  220. 7.1.1Chlortetracycline Repealed
  221. 7.1.2Oxytetracycline and tetracycline Repealed
  222. 7.2Inoculation of the culture medium Repealed
  223. 7.3Seeding Repealed
  224. 7.4Incubation Repealed
  225. 7.5Measurement of growth Repealed
  226. 8Calculation of results Repealed
  227. 9Repeatability Repealed
  228. 3DETERMINATION OF OLEANDOMYCIN Repealed
  229. 1Purpose and scope Repealed
  230. 2Principle Repealed
  231. Micro-organism: B. cereus K 250 TR (resistant to tetracyclines) Repealed
  232. 3.1Maintenance of the parent strain Repealed
  233. 3.2Preparation of the spore suspension Repealed
  234. Culture media and reagents Repealed
  235. 4.1Medium for maintenance of the parent strain Repealed
  236. 4.2Basic medium for the determination Repealed
  237. 4.3Division 4.3 Repealed
  238. 4.4Division 4.4 Repealed
  239. 4.5Division 4.5 Repealed
  240. 4.6Division 4.6 Repealed
  241. 4.7Extraction solution Repealed
  242. 4.8Division 4.8 Repealed
  243. 5Standard solution Repealed
  244. 6Extraction Repealed
  245. Determination method Repealed
  246. 7.1Inoculation of the culture medium Repealed
  247. 7.2Preparation of the trays Repealed
  248. 7.3Incubation Repealed
  249. 8Evaluation Repealed
  250. 9Repeatability Repealed
  251. 4DETERMINATION OF TYLOSIN Repealed
  252. 1Purpose and scope Repealed
  253. 2Principle Repealed
  254. Micro-organism: Sarcina lutea ATCC No 9341 Repealed
  255. 3.1Maintenance of the parent strain Repealed
  256. 3.2Preparation of the bacteria suspension Repealed
  257. Culture media and reagents Repealed
  258. 4.1Basic medium for the determination Repealed
  259. 4.2Phosphate buffer solution, pH 8 Repealed
  260. 4.3Phosphate buffer solution, pH 7 Repealed
  261. 4.4Division 4.4 Repealed
  262. 4.5Division 4.5 Repealed
  263. 4.6Division 4.6 Repealed
  264. 4.7Division 4.7 Repealed
  265. 4.8Division 4.8 Repealed
  266. 5Standard solutions Repealed
  267. 6Extraction Repealed
  268. Determination method Repealed
  269. 7.1Inoculation of the culture medium Repealed
  270. 7.2Preparation of the trays Repealed
  271. 7.3Incubation Repealed
  272. 8Evaluation Repealed
  273. 9Repeatability Repealed
  274. 5DETERMINATION OF VIRGINIAMYCIN Repealed
  275. 1Purpose and scope Repealed
  276. 2Principle Repealed
  277. Micro-organism: Micrococcus luteus ATCC 9341 (NCTC 8340, NCIB 8553) Repealed
  278. 3.1Maintenance of stock culture Repealed
  279. 3.2Preparation of the bacterial suspension Repealed
  280. Culture media and reagents Repealed
  281. 4.1Culture and assay medium Repealed
  282. 4.2Phosphate buffer, pH 6 Repealed
  283. 4.3Division 4.3 Repealed
  284. 4.4Division 4.4 Repealed
  285. 4.5Division 4.5 Repealed
  286. 4.6Division 4.6 Repealed
  287. 4.7Division 4.7 Repealed
  288. 5Standard solutions Repealed
  289. Preparation of the extract and assay solutions Repealed
  290. Extraction Repealed
  291. 6.1.1Products with a virginiamycin content up to 100 mg/kg Repealed
  292. 6.1.2Products with a virginiamycin content greater than 100 mg/kg Repealed
  293. 6.2Assay solutions Repealed
  294. Assay procedure Repealed
  295. 7.1Inoculation of the assay medium Repealed
  296. 7.2Preparation of the plates Repealed
  297. 7.3Incubation Repealed
  298. 8Evaluation Repealed
  299. 9Repeatability Repealed