Division 6.2 Determination

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1. 6.2.1Division 6.2.1
2. 6.2.2Division 6.2.2
3. 6.2.3Division 6.2.3
4. 6.2.4Division 6.2.4
5. 6.2.5Division 6.2.5
6. 6.2.6Division 6.2.6
7. 6.2.7Allow the apparatus to stand until the upper liquid layer has become clear and has distinctly separated from the lower aqueous layer. Alternatively carry out the separation using a suitable centrifuge (5.8).
8. 6.2.8Remove the stopper, rinse it and the inside of the neck of the apparatus with a few millilitres of mixed solvent (4.5) and allow the rinsings to run into the apparatus. Carefully transfer as much as possible of the supernatant layer by decantation or by means of a siphon (5.7) into the prepared flask (6.2.1).
9. 6.2.9Division 6.2.9
10. 6.2.10Division 6.2.10
11. 6.2.11Make a third extraction by repeating the procedure of 6.2.10 but omit the final rinsing (6.2.9).
12. 6.2.12Division 6.2.12
13. 6.2.13Division 6.2.13
14. 6.2.14Division 6.2.14
15. 6.2.15Division 6.2.15
16. 6.2.16Division 6.2.16
17. 6.2.16.1Division 6.2.16.1
18. 6.2.16.2If any insoluble matter is present, or in case of doubt completely extract the fat from the flask by repeated washing with warm light petroleum, allowing the undissolved material to settle before each decantation. Rinse the outside of the neck of the flask three times.