Division 6.1 Preparation of the solution

Weigh as quickly as possible 26 ± 0·002 g of the sample and transfer it quantitatively into a 100 ml volumetric flask (5.5) with approximately 60 ml of water.

Dissolve by swirling but without heating.

Where clarification is necessary, add 0·5 ml of lead acetate reagent (4.1).

Mix the solution by rotating the flask and wash the flask walls, until the volume is such that the meniscus is about 10 mm below the calibration mark.

Place the flask in the water-bath controlled (5.6) at 20 ± 0·1 ^oC until the temperature of the sugar solution is constant.

Eliminate any bubbles formed at the surface of the liquid with a drop of diethyl ether (4.2).

Make up to volume with water.

Stopper and mix thoroughly by inverting the flask at least three times.

Allow to stand for five minutes.