Division 4.2.3.3 Detection

Spray the plate with one of the three reagents (4.2.1.17.1, 4.2.1.17.3 or 4.2.1.17.4). If the plate is sprayed with reagent (4.2.1.17.3), further treat it with bromine vapour (e.g. in a tank containing a small beaker of the reagent (4.2.1.17.2)) until the spots are visible. Detection with the spray reagent (4.2.1.17.4) will be satisfactory only if the drying time for the thin layer has not exceeded 30 minutes.