Division 4.2.3.4 Interpretation
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Compare the Rf values and the colour of the reference solutions with those of the standards. The mean Rf values given below as a rough guide have only a comparative value. They depend upon:
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the state of activation of the thin layer at the time of chromatographing,
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the temperature of the chromatography tank.
Examples of Rf values obtained on a silica gel layer
| Eluting solvents | ||
|---|---|---|
| 4.2.1.16.1 | 4.2.1.16.2 | |
| Mercaptoacetic acid | 0,25 | 0,80 |
| 2-mercaptopropionic acid | 0,40 | 0,95 |
| 2,2'-dithiodi(acetic) acid | 0,00 | 0,35 |
| 3-mercaptopropionic acid | 0,45 | 0,95 |
| 3-mercaptopropane-1,2 diol | 0,45 | 0,35 |