Division 6.4 Methylation of the standard

Cool all reagents and apparatus to between 0 and 4 ^oC for two hours. Into the external compartment of the diazomethane apparatus introduce:

0,2 ml of solution B (6.1.1),

1 ml of ethyl acetate (4 1),

0,1 ml of methanol (4.4).

Continue the methylation as described in 6.3. Inject 1,5 µl of the resultant solution into the chromatograph.