Division 2 Antibiotics as listed below must be added to produce these concentrations in the final diluted semen:F1
United Kingdom
not less than:
-
500 μg streptomycin per ml final dilution,
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500 IU penicillin per ml final dilution,
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150 μg lincomycin per ml final dilution,
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300 μg spectinomycin per ml final dilution.
An alternative combination of antibiotics with an equivalent effect against campylobacters, leptospires and mycoplasmas may be used.
Immediately after their addition the diluted semen must be kept at a temperature of at least 5 o C for a period of not less than 45 minutes.