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Appendix 4 Determination of contamination level in IF and FISH testsF1

United Kingdom

Original text. Later amendments are not shown.

  1. 1..

    Count the mean number of typical fluorescent cells per field of view (c)

  2. 2..

    Calculate the number of typical fluorescent cells per microscope slide window (C)

    C = c × S/s

    where S = surface area of window of multispot slide, and
    s = surface area of objective field.
    s = πi 2 /4G 2 K 2 where i = field coefficient (varies from 8 to 24 depending upon ocular type)
    K = tube coefficient (1 or 1,25)
    G = magnification of objective (100x, 40x etc.).
  3. 3..

    Calculate the number of typical fluorescent cells per ml of resuspended pellet (N)

    N = C × 1 000 /y × F

    where y = volume of resuspended pellet on each window, and
    F = dilution factor of resuspended pellet.

Annotations

Textual amendments

F1

Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot.