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Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot

EU Directive 2006/56 · Adopted 12 June 2006 · Extends to United Kingdom

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Commission Directive 2006/56/EC

of 12 June 2006

amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot

THE COMMISSION OF THE EUROPEAN COMMUNITIES,

Having regard to the Treaty establishing the European Community,

Having regard to Council Directive 93/85/EEC of 4 October 1993f1 on the control of potato ring rot, and in particular Article 12 thereof,

Whereas:

(1)

One of the important organisms harmful to potatoes is Clavibacter michiganensis (Smith) Davis et al. ssp. sepedonicus (Spieckermann et Kotthoff) Davis et al., the pathogenic agent of the potato ring rot disease (hereinafter referred to as ‘the organism’).

(2)

The organism still occurs in some parts of the Community.

(3)

Council Directive 93/85/EEC has laid down detailed measures to be taken within the Member States against the organism in order to locate it and determine its distribution; prevent its occurrence and spread; and if found, to prevent its spread and to control it with the aim of eradication.

(4)

Since then, there have been significant developments in the understanding of the biology, the detection and identification procedures of the organism; moreover practical experiences gained in controlling the organism calls for review of several technical provisions related to control measures.

(5)

As a result of such developments, it appears necessary to review and update the measures included in the Annexes to Directive 93/85/EEC.

(6)

As regards the detection and identification procedures, recently developed procedures like fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) are incorporated as well as improvements of various technical elements of the current detection and identification procedure.

(7)

As regards the technical elements of the control measures, improved provisions are made for: the way of conservation of tested samples in order to ensure trace back of the organism, the elements needed to determine the extent of the probable contamination, the details of the notification of any confirmed presence of the organism and of the relevant contaminated zone, measures to implement in places of production designated as contaminated and within the demarcated zones.

(8)

The measures provided for in this Directive are in accordance with the opinion of the Standing Committee on Plant Health,

HAS ADOPTED THIS DIRECTIVE:

Annotations to the whole text

Footnotes

f1

OJ L 259, 18.10.1993, p. 2.

Contents

  1. 1Article 1
  2. 2Article 2
  3. 3Article 3
  4. 4Article 4
  5. Signature
  6. Annex I: TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al.
  7. Division
  8. GENERAL PRINCIPLES
  9. FLOW CHART DIAGRAM PRESENTATION
  10. 1.1Detection scheme for the diagnosis of Ring Rot in potato tubers and potato plants with symptoms of ring rot
  11. Scheme for detection and identification of Clavibacter michiganensis ssp. sepedonicus in samples of asymptomatic potato tubers
  12. Principle
  13. 1.3Scheme for detection and identification of Clavibacter michiganensis ssp. sepedonicus in samples of asymptomatic potato plants
  14. VISUAL EXAMINATION FOR RING ROT SYMPTOMS
  15. 2.1Potato plants
  16. 2.2Potato tubers
  17. SAMPLE PREPARATION
  18. Potato tubers
  19. Note:
  20. Division
  21. 3.1.1Division 3.1.1
  22. Note:
  23. 3.1.2Division 3.1.2
  24. 3.1.3Division 3.1.3
  25. Note:
  26. 3.1.4Division 3.1.4
  27. 3.1.5Division 3.1.5
  28. 3.1.6Division 3.1.6
  29. 3.1.7Division 3.1.7
  30. Potato plants
  31. Note:
  32. 3.2.1Division 3.2.1
  33. 3.2.2Division 3.2.2
  34. 3.2.3Division 3.2.3
  35. 3.2.4Division 3.2.4
  36. 3.2.5Division 3.2.5
  37. IF TEST
  38. Principle
  39. Note:
  40. 4.1Division 4.1
  41. 4.2Division 4.2
  43. Division
  44. 4.3.1Division 4.3.1
  45. 4.3.2Division 4.3.2
  46. 4.3.3Division 4.3.3
  47. 4.3.4Division 4.3.4
  48. 4.3.5Division 4.3.5
  49. 4.3.6Division 4.3.6
  50. 4.3.7Division 4.3.7
  52. Division
  53. 4.4.1Division 4.4.1
  54. 4.4.2Division 4.4.2
  55. 4.4.3Division 4.4.3
  56. 4.4.4Division 4.4.4
  57. 4.4.5Division 4.4.5
  58. FISH TEST
  59. Principle
  60. Note:
  61. Potato extract fixation
  62. Division
  63. 5.1.1Division 5.1.1
  64. 5.1.2Division 5.1.2
  65. 5.1.3Division 5.1.3
  66. 5.1.4Division 5.1.4
  67. 5.1.5Division 5.1.5
  68. 5.1.6Division 5.1.6
  69. Pre-hybridisation and hybridisation
  70. 5.2.1Division 5.2.1
  71. 5.2.2Division 5.2.2
  72. 5.2.3Division 5.2.3
  73. 5.2.4Division 5.2.4
  74. 5.2.5Division 5.2.5
  75. 5.2.6Division 5.2.6
  76. 5.2.7Division 5.2.7
  77. 5.2.8Division 5.2.8
  78. 5.2.9Division 5.2.9
  79. 5.2.10Division 5.2.10
  80. 5.2.11Division 5.2.11
  81. Reading the FISH test
  82. 5.3.1Division 5.3.1
  83. 5.3.2Division 5.3.2
  84. 5.3.3Division 5.3.3
  85. 5.3.4Division 5.3.4
  86. 5.3.5Division 5.3.5
  87. 5.3.6Division 5.3.6
  88. PCR TEST
  89. Principles
  90. Note:
  91. DNA purification methods
  92. Division
  93. 6.1.(a)Method according to Pastrik (2000)
  94. 6.1.(b)Other methods
  95. PCR
  96. 6.2.1Division 6.2.1
  97. 6.2.2Division 6.2.2
  98. 6.2.3Division 6.2.3
  99. 6.2.4Division 6.2.4
  100. 6.2.5Division 6.2.5
  101. Analysis of the PCR product
  102. 6.3.1Division 6.3.1
  103. 6.3.2Division 6.3.2
  104. 6.3.3Division 6.3.3
  105. 6.3.4Division 6.3.4
  106. Note:
  107. BIOASSAY TEST
  108. Note:
  109. Division
  110. 7.1Division 7.1
  111. 7.2Division 7.2
  113. Division
  114. 7.3.1Division 7.3.1
  115. 7.3.2Division 7.3.2
  116. 7.3.3Division 7.3.3
  117. 7.4Division 7.4
  118. 7.5Division 7.5
  119. 7.6Division 7.6
  120. 7.7Division 7.7
  121. 7.8Division 7.8
  122. 7.9Division 7.9
  123. 7.10Division 7.10
  124. ISOLATION OF C. M. SUBSP. SEPEDONICUS
  125. Note:
  126. Division
  127. Selective plating
  128. 8.1.1Division 8.1.1
  129. 8.1.2Division 8.1.2
  130. Note:
  131. 8.1.3Division 8.1.3
  132. 8.1.4Division 8.1.4
  133. Purification of suspicious colonies
  134. Note:
  135. 8.2.1Division 8.2.1
  136. 8.2.2Division 8.2.2
  137. 8.2.3Division 8.2.3
  138. IDENTIFICATION
  139. Division
  140. 9.1Nutritional and enzymatic identification tests
  141. 9.2IF-test
  142. 9.3PCR test
  143. 9.4FISH test
  144. 9.5Fatty acid profiling (FAP)
  145. 9.6BOX-PCR
  146. CONFIRMATION TEST
  147. Division
  148. 10.1Division 10.1
  149. 10.2Division 10.2
  150. 10.3Division 10.3
  151. 10.4Division 10.4
  152. 1Laboratories involved in optimisation and validation of protocols
  153. 2Preparation of positive and negative controls for the core screening tests PCR/IF and FISH
  154. 3Buffers for test procedures
  155. 4Determination of contamination level in IF and FISH tests
  156. 5Media for isolation and culture of C. m. subsp. sepedonicus
  157. 6Validated PCR protocol and reagents
  158. 7Validated reagents for FISH test
  159. 8Eggplant culture
  160. 9Gram stain procedure (Hucker's modification) (Doetsch, 1981)
  161. Annex IIAnnex II
  162. Annex IIIAnnex III
  163. Annex IVAnnex IV
  164. Annex VAnnex V