Division 8.2.1

Streak C. m. subsp. sepedonicus –like colonies on to one of the following media: (formulae are given in Appendix 5):

nutrient dextrose agar (for use in subculturing only),

yeast peptone glucose agar,

yeast extract mineral salts agar.

Incubate at 21 °C to 24 °C for up to 10 days.

C. m. subsp. sepedonicus is slow-growing, usually producing pin-point, cream, domed colonies within 10 days. Photos of typical colonies of C. m. subsp. sepedonicus (see web site http://forum.europa.eu.int/Public/irc/sanco/Home/main).