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Annex I TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al.

United Kingdom

Original text. Later amendments are not shown.

  1. Division
  2. GENERAL PRINCIPLES
  3. 1FLOW CHART DIAGRAM PRESENTATION
  4. 2VISUAL EXAMINATION FOR RING ROT SYMPTOMS
  5. 3SAMPLE PREPARATION
  6. 4IF TEST
  7. 5FISH TEST
  8. 6PCR TEST
  9. 7BIOASSAY TEST
  10. 8ISOLATION OF C. M. SUBSP. SEPEDONICUS
  11. 9IDENTIFICATION
  12. 10CONFIRMATION TEST
  13. 1Laboratories involved in optimisation and validation of protocols
  14. 2Preparation of positive and negative controls for the core screening tests PCR/IF and FISH
  15. 3Buffers for test procedures
  16. 4Determination of contamination level in IF and FISH tests
  17. 5Media for isolation and culture of C. m. subsp. sepedonicus
  18. 6Validated PCR protocol and reagents
  19. 7Validated reagents for FISH test
  20. 8Eggplant culture
  21. 9Gram stain procedure (Hucker's modification) (Doetsch, 1981)