Division 3.1.6

Resuspend the pellet in 1,5 ml pellet buffer (Appendix 3). Use 500 µl to test for C. m. subsp. sepedonicus, 500 µl for Ralstonia solanacearum and 500 µl for reference purposes. Add sterile glycerol to final concentration of 10 to 25 % (v/v) to the 500 µl of the reference aliquot and to the remaining test aliquot, vortex and store at -16 to -24 °C (weeks) or at 68 to -86 °C (months). Preserve the test aliquots at 4 to 10 °C during testing.

Repeated freezing and thawing is not advisable.

If transport of the extract is required, ensure delivery in a cool box within 24 to 48 hours.