Division 7.9

As soon as symptoms in eggplants are observed reisolation should be performed, using sections of wilted leaf tissue or stem tissue from plants (see 3.1. 3 for tissue maceration). Surface disinfect the eggplant leaves and stems by wiping with 70 % ethanol. Perform an IF test or PCR on the eggplant sap and isolate on to suitable (selective) media (see section 8). A Gram stain (Appendix 9) may also be prepared. Identify purified cultures of presumptive C. m. subsp. sepedonicus and confirm pathogenicity (see section 9 and 10).