Division 7.7 F1

Incubate plants in quarantine facilities for up to four weeks at 18 to 24 °C. Incubate plants with sufficient light and high humidity (70 to 80 %) and water to prevent water logging or wilting through water deficiency. C. m. sepedonicus cells are killed at temperatures above 30 °C and the optimum temperature is 21 °C. To avoid contamination incubate positive control and negative control plants on clearly separated benches in a glasshouse or growth chamber or, in case space is limited, ensure strict separation between treatments. If plants for different samples must be incubated close together, separate them with appropriate screens. When fertilising, watering, inspecting and any other manipulations take great care to avoid cross-contamination. It is essential to keep glasshouses and growth chambers free of all insect pests since they may transmit the bacterium from sample to sample.