Division 7.3 PCR methodsF1

Specific PCR primers (Pastrik et al , 2002; see Appendix 6) can be used to differentiate strains belonging to division 1 (biovars 3, 4 and 5) and division 2 (biovars 1, 2A and 2T) of R. solanacearum , as originally defined by RFLP analysis (Cook et al. , 1989) and 16S rDNA sequencing (Taghavi et al. , 1996).