Division 1.4.2.4 Initial test (In vivo eye irritation/corrosion test using one animal)

As articulated in the sequential testing strategy (see Annex 1), it is strongly recommended that the in vivo test be performed initially using one animal.

If the results of this test indicate the substance to be corrosive or a severe irritant to the eye using the procedure described, further testing for ocular irritancy should not be performed.