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Commission Directive 2004/73/EC of 29 April 2004 adapting to technical progress for the twenty-ninth time Council Directive 67/548/EEC on the approximation of the laws, regulations and administrative provisions relating to the classification, packaging and labelling of dangerous substances (Text with EEA relevance)

EU Directive 2004/73 · Adopted 29 April 2004 · Extends to United Kingdom

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Commission Directive 2004/73/EC

of 29 April 2004

adapting to technical progress for the twenty-ninth time Council Directive 67/548/EEC on the approximation of the laws, regulations and administrative provisions relating to the classification, packaging and labelling of dangerous substances

(Text with EEA relevance)

THE COMMISSION OF THE EUROPEAN COMMUNITIES,

Having regard to the Treaty establishing the European Community,

Having regard to Council Directive 67/548/EEC of 27 June 1967 on the approximation of the laws, regulations and administrative provisions relating to the classification, packaging and labelling of dangerous substancesf1, and in particular Article 28 thereof,

Whereas:

(1)

Annex I to Directive 67/548/EEC contains a list of dangerous substances, together with particulars of the classification and labelling of each substance. That list needs to be updated to include further notified new substances and further existing substances as well adapting the existing entries to technical progress such as setting environmental concentration limits for certain substances. Accordingly it is also necessary to delete entries for certain substances and to split some entries because the classification no longer applies to all the substances under those entries. The labelling of substances containing 1,3-butadiene should be changed in order to reflect that that substance will be classified as a mutagen by the present Directive.

(2)

Annex V to Directive 67/548/EEC lays down the methods for the determination of the physicochemical properties, toxicity and ecotoxicity of substances and preparations. It is appropriate to amend that Annex in order to obtain a reduction to a minimum of the number of animals used for experimental purposes, in accordance with Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposesf2. The methods for sub-chronic oral toxicity in Chapters B.1, B.4, B.5, B.31 and B.35 should be revised accordingly. Furthermore, Chapter B.42 should be added to Annex V in order to make available a refined method on sub-chronic oral toxicity. Finally, Chapter A.21 on physico-chemical properties, Chapter B.43 on sub-chronic oral toxicity and Chapters C.21 to C.24 on environmental toxicity should be added in order to allow for the determination of properties which are not yet sufficiently covered by the methods in Annex V.

(3)

The measures provided for in this Directive are in accordance with the opinion of the Committee on the Adaptation to Technical Progress of the Directives for the Elimination of Technical Barriers to Trade with Dangerous Substances and Preparations,

HAS ADOPTED THIS DIRECTIVE:

Annotations to the whole text

Footnotes

f1

OJ 196,16.8.1967, p. 1. Directive as last amended by Commission Directive 2001/59/EC (OJ L 225, 6.8.2001, p. 1)

f2

OJ L 358, 18.12.1986, p. 1. Directive as last amended by Directive 2003/65/EC of the European Parliament and of the Council (OJ L 230,16.9.2003, p. 32).

Contents

  1. 1Article 1
  2. 2Article 2
  3. 3Article 3
  4. 4Article 4
  5. Signature
  6. Annex 1AAnnex 1A
  7. Note K:
  8. Annex 2AAnnex 2A
  9. OXIDIZING PROPERTIES (LIQUIDS)
  10. METHOD
  11. 1.1INTRODUCTION
  12. 1.2DEFINITIONS AND UNITS
  13. 1.3REFERENCE SUBSTANCE
  14. 1.4PRINCIPLE OF THE TEST METHOD
  15. 1.5QUALITY CRITERIA
  16. DESCRIPTION OF THE METHOD
  17. Preparation
  18. 1.6.1.1Combustible substance
  19. 1.6.1.2Apparatus
  20. 1.6.2Performance of the test
  21. 2DATA
  22. 2.1TREATMENT OF RESULTS
  23. REPORT
  24. 3.1TEST REPORT
  25. 3.2INTERPRETATION OF THE RESULTS
  26. REFERENCES
  27. (1)Division (1)
  28. Annex 2B
  29. ACUTE ORAL TOXICITY - FIXED DOSE PROCEDURE
  30. 1METHOD
  31. 1.1INTRODUCTION
  32. 1.2DEFINITIONS
  33. 1.3PRINCIPLE OF THE TEST METHOD
  34. DESCRIPTION OF THE TEST METHOD
  35. 1.4.1Selection of animal species
  36. 1.4.2Housing and feeding conditions
  37. 1.4.3Preparation of animals
  38. 1.4.4Preparation of doses
  39. PROCEDURE
  40. 1.5.1Administration of doses
  41. 1.5.2Sighting study
  42. 1.5.3Main study
  43. 1.6OBSERVATIONS
  44. 1.6.1Body weight
  45. 1.6.2Pathology
  46. 2DATA
  47. REPORTING
  48. 3.1Test report
  49. REFERENCES
  50. (1)Division (1)
  51. (2)Division (2)
  52. (3)Division (3)
  53. (4)Division (4)
  54. (5)Division (5)
  55. (6)Division (6)
  56. (7)Division (7)
  57. (8)Division (8)
  58. (9)Division (9)
  59. (10)Division (10)
  60. (11)Division (11)
  61. ANNEX 1: FLOW CHART FOR THE SIGHTING STUDY
  62. ANNEX 1: FLOW CHART FOR THE SIGHTING STUDY
  63. ANNEX 2: FLOW CHART FOR THE MAIN STUDY
  64. ANNEX 2: FLOW CHART FOR THE MAIN STUDY
  65. ANNEX 3
  66. CRITERIA FOR CLASSIFICATION OF TEST SUBSTANCES WITH EXPECTED LD50 VALUES EXCEEDING 2000 MG/KG WITHOUT THE NEED FOR TESTING.
  67. TESTING AT DOSES ABOVE 2000 MG/KG
  68. Sighting Study
  69. Main Study
  70. ANNEX 4:TEST METHOD B.1 bis - Guidance on classification according to the EU scheme to cover the transition period until full implementation of the Globally Harmonised Classification System (GHS) (taken from reference (8))
  71. ANNEX 4:TEST METHOD B. 1 bis - Guidance on classification according to the EU scheme to cover the transition period until full implementation of the Globally Harmonised Classification System (GHS) (taken from reference (8))
  72. Annex 2C
  73. ACUTE ORAL TOXICITY - ACUTE TOXIC CLASS METHOD
  74. 1METHOD
  75. 1.1INTRODUCTION
  76. 1.2DEFINITIONS
  77. 1.3PRINCIPLE OF THE TEST
  78. DESCRIPTION OF THE METHOD
  79. 1.4.1Selection of animal species
  80. 1.4.2Housing and feeding conditions
  81. 1.4.3Preparation of animals
  82. 1.4.4Preparation of doses
  83. PROCEDURE
  84. 1.5.1Administration of doses
  85. 1.5.2Number of animals and dose levels
  86. 1.5.3Limit test
  87. 1.6OBSERVATIONS
  88. 1.6.1Body weight
  89. 1.6.2Pathology
  90. 2DATA
  91. REPORTING
  92. 3.1Test report
  93. REFERENCES
  94. (1)Division (1)
  95. (2)Division (2)
  96. (3)Division (3)
  97. (4)Division (4)
  98. (5)Division (5)
  99. (6)Division (6)
  100. (7)Division (7)
  101. (8)Division (8)
  102. (9)Division (9)
  103. (10)Division (10)
  104. (11)Division (11)
  105. (12)Division (12)
  106. PROCEDURE TO BE FOLLOWED FOR EACH OF THE STARTING DOSES
  107. GENERAL REMARKS
  108. ANNEX 2
  109. CRITERIA FOR CLASSIFICATION OF TEST SUBSTANCES WITH EXPECTED LD50 VALUES EXCEEDING 2000 MG/KG WITHOUT THE NEED FOR TESTING
  110. TESTING AT DOSES ABOVE 2000 MG/KG
  111. Annex 2D
  112. ACUTE TOXICITY: DERMAL IRRITATION/CORROSION
  113. 1METHOD
  114. 1.1INTRODUCTION
  115. 1.2DEFINITIONS
  116. 1.3PRINCIPLE OF THE TEST METHOD
  117. DESCRIPTION OF THE TEST METHOD
  118. Preparation for the in vivo test
  119. 1.4.1.1Selection of animal species
  120. 1.4.1.2Preparation of the animals
  121. 1.4.1.3Housing and feeding conditions
  122. Test procedure
  123. 1.4.2.1Application of the test substance
  124. 1.4.2.2Dose level
  125. 1.4.2.3Initial test (In vivo dermal irritation/corrosion test using one animal)
  126. 1.4.2.4Confirmatory test (In vivo dermal irritation test with additional animals)
  127. 1.4.2.5Observation period
  128. 1.4.2.6Clinical observations and grading of skin reactions
  129. DATA
  130. 2.1PRESENTATION OF RESULTS
  131. 2.2EVALUATION OF RESULTS
  132. REPORTING
  133. 3.1TEST REPORT
  134. REFERENCES
  135. (1)Division (1)
  136. (2)Division (2)
  137. (3)Division (3)
  138. (4)Division (4)
  139. (5)Division (5)
  140. (5a)Division (5a)
  141. (6)Division (6)
  142. (7)Division (7)
  143. (8)Division (8)
  144. (9)EPA (1990). Atlas of Dermal Lesions, (20T-2004). United States Environmental Protection Agency, Office of Pesticides and Toxic Substances, Washington, DC, August 1990.
  145. TABLE I:GRADING OF SKIN REACTIONS
  146. A Sequential Testing Strategy for Dermal Irritation and Corrosion
  147. GENERAL CONSIDERATIONS
  148. DESCRIPTION OF THE EVALUATION AND TESTING STRATEGY
  149. REFERENCES
  150. (1)Division (1)
  151. (2)Division (2)
  152. (3)Division (3)
  153. (4)Division (4)
  154. (5)Division (5)
  155. (6)Division (6)
  156. (7)Division (7)
  157. FIGURETESTING AND EVALUATION STRATEGY FOR DERMAL IRRITATION /CORROSION
  158. Annex 2E
  159. ACUTE TOXICITY: EYE IRRITATION/CORROSION
  160. 1METHOD
  161. 1.1INTRODUCTION
  162. 1.2DEFINITIONS
  163. 1.3PRINCIPLE OF THE TEST METHOD
  164. DESCRIPTION OF THE TEST METHOD
  165. Preparation for the in vivo test
  166. 1.4.1.1Selection of species
  167. 1.4.1.2Preparation of animals
  168. 1.4.1.3Housing and feeding conditions
  169. Test procedure
  170. 1.4.2.1Application of the test substance
  171. 1.4.2.2Irrigation
  172. 1.4.2.3Dose level
  173. 1.4.2.4Initial test (In vivo eye irritation/corrosion test using one animal)
  174. 1.4.2.5Local anaesthetics
  175. 1.4.2.6Confirmatory test (In vivo eye irritation test with additional animals)
  176. 1.4.2.7Observation period
  177. DATA
  178. 2.2EVALUATION OF RESULTS
  179. REPORTING
  180. 3.1TEST REPORT
  181. 3.2INTERPRETATION OF THE RESULTS
  182. REFERENCES
  183. (1)Division (1)
  184. (2)Division (2)
  185. (3)Division (3)
  186. (4)Division (4)
  187. (5)Division (5)
  188. (6)Division (6)
  189. (6a)Division (6a)
  190. (7)Division (7)
  191. (8)Division (8)
  192. (9)Division (9)
  193. (10)Division (10)
  194. TABLE I: GRADING OF OCULAR LESIONS
  195. A Sequential Testing Strategy for Eye Irritation and Corrosion
  196. GENERAL CONSIDERATIONS
  197. DESCRIPTION OF THE STEPWISE TESTING STRATEGY
  198. REFERENCES
  199. (1)Division (1)
  200. (2)Division (2)
  201. (3)Division (3)
  202. (4)Division (4)
  203. (5)Division (5)
  204. (6)Division (6)
  205. (7)Division (7)
  206. (8)Division (8)
  207. (9)Division (9)
  208. FIGURETESTING AND EVALUATION STRATEGY FOR EYE IRRITATION/CORROSION
  209. Annex 2F
  210. PRENATAL DEVELOPMENTAL TOXICITY STUDY
  211. 1METHOD
  212. 1.1INTRODUCTION
  213. 1.2DEFINITIONS
  214. 1.3REFERENCE SUBSTANCE
  215. 1.4PRINCIPLE OF THE TEST METHOD
  216. DESCRIPTION OF THE TEST METHOD
  217. 1.5.1Selection of animal species
  218. 1.5.2Housing and feeding conditions
  219. 1.5.3Preparation of the animals
  220. PROCEDURE
  221. 1.6.1Number and sex of animals
  222. 1.6.2Preparation of doses
  223. 1.6.3Dosage
  224. 1.6.4Limit test
  225. 1.6.5Administration of doses
  226. 1.6.6Observations of the dams
  227. 1.6.7Body weight and food consumption
  228. 1.6.8Post-mortem examination
  229. 1.6.9Examination of uterine contents
  230. 1.6.10Examination of foetuses
  231. DATA
  232. 2.1TREATMENT OF RESULTS
  233. 2.2EVALUATION OF RESULTS
  234. 2.3INTERPRETATION OF RESULTS
  235. REPORTING
  236. TEST REPORT
  237. REFERENCES
  238. (1)Division (1)
  239. (2)Division (2)
  240. (3)Division (3)
  241. (4)Division (4)
  242. (5)Division (5)
  243. (6)Division (6)
  244. (7)Division (7)
  245. (8)Division (8)
  246. (9)Division (9)
  247. (10)Division (10)
  248. (11)Division (11)
  249. (12)Division (12)
  250. (13)Division (13)
  251. (14)Division (14)
  252. (15)Division (15)
  253. (16)Division (16)
  254. (17)Division (17)
  255. (18)Division (18)
  256. (19)Division (19)
  257. (20)Division (20)
  258. (21)Division (21)
  259. (22)Division (22)
  260. (23)Division (23)
  261. (24)Division (24)
  262. (25)Division (25)
  263. (26)Division (26)
  264. (27)Division (27)
  265. (28)Division (28)
  266. Annex 2G
  267. TWO-GENERATION REPRODUCTION TOXICITY STUDY
  268. 1METHOD
  269. 1.1INTRODUCTION
  270. 1.2PRINCIPLE OF THE TEST METHOD
  271. DESCRIPTION OF THE TEST METHOD
  272. 1.3.1Selection of animal species
  273. 1.3.2Housing and feeding conditions
  274. 1.3.3Preparation of animals
  275. PROCEDURE
  276. 1.4.1Number and sex of animals
  277. 1.4.2Preparation of Doses
  278. 1.4.3Dosage
  279. 1.4.4Limit test
  280. 1.4.5Administration of doses
  281. 1.4.6Experimental schedules
  282. Mating procedure
  283. 1.4.7.1Parental (P) mating
  284. 1.4.7.2F1 mating
  285. 1.4.7.3Second mating
  286. 1.4.7.4Litter size
  287. OBSERVATIONS
  288. 1.5.1Clinical observations
  289. 1.5.2Body weight and food/water consumption of parent animals
  290. 1.5.3Oestrus cycle
  291. 1.5.4Sperm parameters
  292. 1.5.5Offspring
  293. 1.5.6Gross necropsy
  294. 1.5.7Organ weights
  295. Histopathology
  296. 1.5.8.1Parental Animals
  297. 1.5.8.2Weanlings
  298. DATA
  299. 2.1TREATMENT OF RESULTS
  300. 2.2EVALUATION OF RESULTS
  301. 2.3INTERPRETATION OF RESULTS
  302. REPORTING
  303. TEST REPORT
  304. REFERENCES
  305. (1)Division (1)
  306. (2)Division (2)
  307. (3)Division (3)
  308. (4)Division (4)
  309. (5)Division (5)
  310. (6)Division (6)
  311. (7)Division (7)
  312. (8)Division (8)
  313. (9)Division (9)
  314. (10)Division (10)
  315. (11)Division (11)
  316. (12)Division (12)
  317. (13)Division (13)
  318. (14)Division (14)
  319. (15)Division (15)
  320. (16)Division (16)
  321. (17)Division (17)
  322. (18)Division (18)
  323. (19)Division (19)
  324. (20)Division (20)
  325. (21)Division (21)
  326. (22)Division (22)
  327. (23)Division (23)
  328. Annex 2H
  329. SKIN SENSITISATION: LOCAL LYMPH NODE ASSAY
  330. 1METHOD
  331. 1.1INTRODUCTION
  332. 1.2PRINCIPLE OF THE TEST METHOD
  333. DESCRIPTION OF THE TEST METHOD
  334. Preparations
  335. 1.3.1.1Housing and feeding conditions
  336. 1.3.1.2Preparation of animals
  337. Test Conditions
  338. 1.3.2.1Experimental animals
  339. 1.3.2.2Reliability check
  340. 1.3.2.3Number of animals, dose levels and vehicle selection.
  341. Test procedure
  342. 1.3.3.1Experimental schedule
  343. 1.3.3.2Preparation of cell suspensions
  344. 1.3.3.3Determination of cell proliferation (incorporated radioactivity)
  345. 1.3.3.4Observations
  346. 1.3.4Calculation of results
  347. 2DATA
  348. REPORTING
  349. TEST REPORT
  350. REFERENCES
  351. 1Division 1
  352. 2Division 2
  353. 3Division 3
  354. 4Division 4
  355. 5Division 5
  356. 6Division 6
  357. 7Division 7
  358. 8Division 8
  359. 9Division 9
  360. 10Division 10
  361. 11Division 11
  362. 12Division 12
  363. 13Division 13
  364. 14Division 14
  365. NEUROTOXICITY STUDY IN RODENTS
  366. 1METHOD
  367. 1.1INTRODUCTION
  368. 1.2DEFINITIONS
  369. 1.3PRINCIPLE OF THE TEST METHOD
  370. DESCRIPTION OF THE TEST METHOD
  371. 1.4.1Selection of animal species
  372. 1.4.2Housing and feeding conditions
  373. 1.4.3Preparation of animals
  374. 1.4.4Route of administration and preparation of doses
  375. PROCEDURES
  376. 1.5.1Number and sex animals
  377. 1.5.2Treatment and control group
  378. 1.5.3Reliability check
  379. 1.5.4Dose selection
  380. 1.5.5Limit test
  381. 1.5.6Administration of doses
  382. OBSERVATION
  383. 1.6.1Frequency of observations and tests
  384. 1.6.1.1Observations of general health condition and mortality/morbidity
  385. 1.6.1.2Detailed clinical observations
  386. 1.6.1.3Functional tests
  387. 1.6.2Body weight and food/water consumption
  388. 1.6.3Ophthalmology
  389. 1.6.4Haematology and clinical biochemistry
  390. 1.6.5Histopathology
  391. DATA
  392. 2.1TREATMENT OF RESULTS
  393. 2.2EVALUATION AND INTERPRETATION OF RESULTS
  394. 3REPORTING
  395. 4REFERENCES
  396. 1Division 1
  397. 2Division 2
  398. 3Division 3
  399. 4Division 4
  400. 5Division 5
  401. 6Division 6
  402. 7Division 7
  403. 8Division 8
  404. 9Division 9
  405. 10Division 10
  406. 11Division 11
  407. 12Division 12
  408. 13Division 13
  409. 14Division 14
  410. 15Division 15
  411. 16Division 16
  412. 17Division 17
  413. 18Division 18
  414. Annex 2I
  415. SOIL MICROORGANISMS: NITROGEN TRANSFORMATION TEST
  416. 1METHOD
  417. 1.1INTRODUCTION
  418. 1.2DEFINITIONS
  419. 1.3REFERENCE SUBSTANCES
  420. 1.4PRINCIPLE OF THE TEST METHOD
  421. 1.5VALIDITY OF THE TEST
  422. DESCRIPTION OF THE TEST METHOD
  423. 1.6.1Apparatus
  424. 1.6.2Selection and number of soils
  425. Collection and storage of soil samples
  426. 1.6.3.1Collection
  427. 1.6.3.2Storage
  428. Handling and preparation of soil for the test
  429. 1.6.4.1Pre-incubation
  430. 1.6.4.2Physical-chemical characteristics
  431. 1.6.4.3Amendment with organic substrate
  432. 1.6.5Preparation of the test substance for the application to soil
  433. 1.6.6Test concentrations
  434. PERFORMANCE OF THE TEST
  435. Conditions of exposure
  436. 1.7.1.1Treatment and control
  437. 1.7.1.2Incubation of soil samples
  438. 1.7.1.3Test conditions and duration
  439. Sampling and analysis of soils
  440. 1.7.2.1Soil sampling schedule
  441. 1.7.2.2Analysis of soil samples
  442. DATA
  443. 2.1TREATMENT OF RESULTS
  444. 2.2INTERPRETATION OF RESULTS
  445. 3REPORTING
  446. REFERENCES
  447. (1)Division (1)
  448. (2)Division (2)
  449. (3)Division (3)
  450. (4)Division (4)
  451. (5)Division (5)
  452. (6)Division (6)
  453. (7)Division (7)
  454. (8)Division (8)
  455. (9)Division (9)
  456. (10)Division (10)
  457. (11)Division (11)
  458. (12)Division (12)
  459. SOIL MICROORGANISMS: CARBON TRANSFORMATION TEST
  460. 1METHOD
  461. 1.1INTRODUCTION
  462. 1.2DEFINITIONS
  463. 1.3REFERENCE SUBSTANCES
  464. 1.4PRINCIPLE OF THE TEST METHOD
  465. 1.5VALIDITY OF THE TEST
  466. DESCRIPTION OF THE TEST METHOD
  467. 1.6.1Apparatus
  468. 1.6.2Selection and number of soils
  469. Collection and storage of soil samples
  470. 1.6.3.1Collection
  471. 1.6.3.2Storage
  472. Handling and preparation of soil for the test
  473. 1.6.4.1Pre-incubation
  474. 1.6.4.2Physical-chemical characteristics
  475. 1.6.5Preparation of the test substance for the application to soil
  476. 1.6.6Test concentrations
  477. PERFORMANCE OF THE TEST
  478. Conditions of exposure
  479. 1.7.1.1Treatment and control
  480. 1.7.1.2Incubation of soil samples
  481. 1.7.1.3Test conditions and duration
  482. Sampling and analysis of soils
  483. 1.7.2.1Soil sampling schedule
  484. 1.7.2.2Measurement of glucose-induced respiration rates
  485. DATA
  486. 2.1TREATMENT OF RESULTS
  487. 2.2INTERPRETATION OF RESULTS
  488. REPORTING
  489. TEST REPORT
  490. REFERENCES
  491. (1)Division (1)
  492. (2)Division (2)
  493. (3)Division (3)
  494. (4)Division (4)
  495. (5)Division (5)
  496. (6)Division (6)
  497. (7)Division (7)
  498. (8)Division (8)
  499. (9)Division (9)
  500. (10)Division (10)
  501. (11)Division (11)
  502. (12)Division (12)
  503. (13)Division (13)
  504. (14)Division (14)
  505. (15)Division (15)
  506. (16)Division (16)
  507. (17)Division (17)
  508. AEROBIC AND ANAEROBIC TRANSFORMATION IN SOIL
  509. 1METHOD
  510. 1.1INTRODUCTION
  511. 1.2DEFINITIONS
  512. 1.3REFERENCE SUBSTANCES
  513. 1.4APPLICABILITY OF THE TEST
  514. 1.5INFORMATION ON THE TEST SUBSTANCE
  515. 1.6PRINCIPLE OF THE TEST METHOD
  516. QUALITY CRITERIA
  517. 1.7.1Recovery
  518. 1.7.2Repeatability and sensitivity of analytical method
  519. 1.7.3Accuracy of transformation data
  520. DESCRIPTION OF THE TEST METHOD
  521. 1.8.1Equipment and chemical reagents
  522. 1.8.2Test substance application
  523. Soils
  524. 1.8.3.1Soil selection
  525. 1.8.3.2Collection, handling, and storage of soils
  526. PERFORMANCE OF THE TEST
  527. Test conditions
  528. 1.9.1.1Test temperature
  529. 1.9.1.2Moisture content
  530. 1.9.1.3Aerobic incubation conditions
  531. 1.9.1.4Sterile aerobic conditions
  532. 1.9.1.5Anaerobic incubation conditions
  533. 1.9.1.6Paddy incubation conditions
  534. 1.9.1.7Test duration
  535. 1.9.2Performance of the test
  536. 1.9.3Sampling and measurement
  537. 1.9.4Optional tests
  538. DATA
  539. 2.1TREATMENT OF RESULTS
  540. 2.2EVALUATION AND INTERPRETATION OF RESULTS
  541. REPORTING
  542. TEST REPORT
  543. REFERENCES
  544. (1)Division (1)
  545. (2)Division (2)
  546. (3)Division (3)
  547. (4)Division (4)
  548. (5)Division (5)
  549. (6)Division (6)
  550. (7)Division (7)
  551. (8)Division (8)
  552. (9)Division (9)
  553. (10)Division (10)
  554. (11)Division (11)
  555. (12)Division (12)
  556. (13)Division (13)
  557. (14)Division (14)
  558. (15)Division (15)
  559. (16)Division (16)
  560. (17)Division (17)
  561. (18)Division (18)
  562. (19)Division (19)
  563. (20)Division (20)
  564. (21)Division (21)
  565. (22)Division (22)
  566. (23)Division (23)
  567. (24)Division (24)
  568. (25)Division (25)
  569. (26)Division (26)
  570. (27)Division (27)
  571. (28)Division (28)
  572. (29)Division (29)
  573. (30)Division (30)
  574. (31)Division (31)
  575. (32)Division (32)
  576. (33)Division (33)
  577. (34)Division (34)
  578. (35)Division (35)
  579. (36)Division (36)
  580. (37)Division (37)
  581. ANNEX 1WATER TENSION, FIELD CAPACITY (FC) AND WATER HOLDING CAPACITY (WHC)(1)
  582. (1)Division (1)
  583. ANNEX 3
  584. (1)Division (1)
  585. (2)Division (2)
  586. (3)Division (3)
  587. AEROBIC AND ANAEROBIC TRANSFORMATION IN AQUATIC SEDIMENT SYSTEMS
  588. 1METHOD
  589. 1.1INTRODUCTION
  590. 1.2DEFINITIONS
  591. 1.3REFERENCE SUBSTANCES
  592. 1.4INFORMATION ON THE TEST SUBSTANCE
  593. Division
  594. 1.5PRINCIPLE OF THE TEST METHOD
  595. 1.6APPLICABILITY OF THE TEST
  596. QUALITY CRITERIA
  597. 1.7.1Recovery
  598. 1.7.2Repeatability and sensitivity of analytical method
  599. 1.7.3Accuracy of transformation data
  600. DESCRIPTION OF THE METHOD
  601. 1.8.1Test system and apparatus
  602. 1.8.2Selection and number of aquatic sediments
  603. 1.8.2.1Sediment selection
  604. 1.8.2.2Characterisation of water-sediment samples
  605. Collection, Handling and Storage
  606. 1.8.3.1Collection
  607. 1.8.3.2Handling
  608. 1.8.3.3Storage
  609. 1.8.4Preparation of the sediment/water samples for the test
  610. PERFORMANCE OF THE TEST
  611. 1.9.1Test conditions
  612. 1.9.2Treatment and application of test substance
  613. 1.9.3Test duration and sampling
  614. 1.9.4Optional preliminary test
  615. 1.9.5Measurements and analysis
  616. DATA
  617. 2.1TREATMENT OF RESULTS
  618. REPORTING
  619. 3.1TEST REPORT
  620. REFERENCES
  621. (1)Division (1)
  622. (2)Division (2)
  623. (3)Division (3)
  624. (4)Division (4)
  625. (5)Division (5)
  626. (6)Division (6)
  627. (7)Division (7)
  628. (8)Division (8)
  629. (9)Division (9)
  630. (10)Division (10)
  631. (11)Division (11)
  632. (12)Division (12)
  633. (13)Division (13)
  634. (14)Division (14)
  635. (15)Division (15)
  636. (16)Division (16)
  637. (17)Division (17)
  638. (18)Division (18)
  639. (19)Division (19)
  640. (20)Division (20)
  641. (21)Division (21)
  642. (22)Division (22)
  643. (23)Division (23)
  644. (24)Division (24)
  645. (25)Division (25)
  646. (26)Division (26)
  647. (27)Division (27)
  648. (28)Division (28)
  649. (29)Division (29)
  650. (30)Division (30)
  651. (31)Division (31)
  652. (32)Division (32)
  653. (33)Division (33)
  654. (34)Division (34)
  655. (35)Division (35)
  656. (36)Division (36)
  657. (37)Division (37)
  658. GUIDANCE ON THE AEROBIC AND THE ANAEROBIC TEST SYSTEMS
  659. Aerobic test system
  660. Anaerobic test system
  661. ANNEX 2EXAMPLE OF A GAS FLOW-THROUGH APPARATUS