Division 3.1 TEST REPORT

The report must include the following information:

Test substance:

• common name, chemical name, CAS number, structural formula (indicating position of the label(s) when radiolabelled material is used) and relevant physical-chemical properties;

• purity (impurities) of test substance;

• radiochemical purity of labelled chemical and molar activity (where appropriate).

Reference substances:

• chemical name and structure of reference substances used for the characterisation and/or identification of transformation products

Test sediments and waters:

• location and description of aquatic sediment sampling site(s) including, if possible, contamination history;

• all information relating to the collection, storage (if any) and acclimation of water-sediment systems;

• characteristics of the water-sediment samples as listed in Table in section 1.8.2.2.

Test conditions:

• test system used (e.g. flow-through, biometer, way of ventilation, method of stirring, water volume, mass of sediment, thickness of both water and sediment layer, dimension of test vessels, etc.)

• application of test substance to test system: test concentration used, number of replicates and controls mode of application of test substance (e.g. use of solvent if any), etc.

• incubation temperature;

• sampling times;

• extraction methods and efficiencies as well as analytical methods and detection limits;

• methods for characterisation/identification of transformation products;

• deviations from the test protocol or test conditions during the study.

Results:

• raw data figures of representative analyses (all raw data have to be stored in the GLP-archive);

• repeatability and sensitivity of the analytical methods used;

• rates of recovery (% values for a valid study are given in section 1.7.1);

• tables of results expressed as % of the applied dose and in mgkg^-1 in water, sediment and total system (% only) for the test substance and, if appropriate, for transformation products and non-extractable radioactivity;

• mass balance during and at the end of the studies;

• a graphical representation of the transformation in the water and sediment fractions and in total system (including mineralisation);

• mineralisation rates;

• half-life, DT₅₀ and, if appropriate, DT₇₅ and DT₉₀ values for the test substance and, where appropriate, for major transformation products including confidence limits in water, sediment and in total system;

• an assessment of the transformation kinetics of the test substance and, where appropriate, the major transformation products;

• a proposed pathway of transformation, where appropriate;

• discussion of results.