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Division 1.8.1 Equipment and chemical reagents

United Kingdom

Original text. Later amendments are not shown.

Incubation systems consist of static closed systems or suitable flow-through systems (7)(17). Examples of suitable flow-through soil incubation apparatus and biometer-type flask are shown in Figures 1 and 2, respectively. Both types of incubation systems have advantages and limitations (7)(17).

Standard laboratory equipment is required and especially the following:

  • Analytical instruments such as GLC, HPLC, TLC-equipment, including the appropriate detection systems for analysing radiolabelled or non-labelled substances or inverse isotopes dilution method;

  • Instruments for identification purposes (e.g. MS, GC-MS, HPLC-MS, NMR, etc.);

  • Liquid scintillation counter;

  • Oxidiser for combustion of radioactive material;

  • Centrifuge;

  • Extraction apparatus (for example, centrifuge tubes for cold extraction and Soxhlet apparatus for continuous extraction under reflux);

  • Instrumentation for concentrating solutions and extracts (e.g. rotating evaporator);

  • Water bath;

  • Mechanical mixing device (e.g. kneading machine, rotating mixer).

Chemical reagents used include, for example:

  • NaOH, analytical grade, 2 mol dm--3, or other appropriate base (e.g. KOH, ethanolamine);

  • H2SO4, analytical grade, 0.05 mol dm--3;

  • Ethylene glycol, analytical grade;

  • Solid absorption materials such as soda lime and polyurethane plugs;

  • Organic solvents, analytical grade, such as acetone, methanol, etc.;

  • Scintillation liquid.