Division TEST REPORT

The test report must include:

Test substance:

• common name, chemical name, CAS number, structural formula (indicating position of label(s) when radiolabelled material is used) and relevant physical-chemical properties (see section 1.5);

• purity (impurities) of test substance;

• radiochemical purity of labelled chemical and specific activity (where appropriate);

Reference substances:

• chemical name and structure of reference substances used for the characterisation and/or identification of transformation product;

Test soils:

• details of collection site;

• date and procedure of soil sampling;

• properties of soils, such as pH, organic carbon content, texture (% sand, % silt, % clay), cation exchange capacity, bulk density, water retention characteristic, and microbial biomass;

• length of soil storage and storage conditions (if stored);

Test conditions:

• dates of the performance of the studies;

• amount of test substance applied;

• solvents used and method of application for the test substance;

• weight of soil treated initially and sampled at each interval for analysis;

• description of the incubation system used;

• air flow rates (for flow-through systems only);

• temperature of experimental set-up;

• soil moisture content during incubation;

• microbial biomass initially, during and at the end of the aerobic studies;

• pH, oxygen concentration and redox potential initially, during and at the end of the anaerobic and paddy studies;

• method(s) of extraction;

• methods for quantification and identification of the test substance and major transformation products in soil and absorption materials;

• number of replicates and number of controls.

Results:

• result of microbial activity determination;

• repeatability and sensitivity of the analytical methods used;

• rates of recovery (% values for a valid study are given in section 1.7.1);

• tables of results expressed as % of applied initial dose and, where appropriate, as mgkg-¹ soil (on a dry weight basis);

• mass balance during and at the end of the studies;

• characterisation of non-extractable (bound) radioactivity or residues in soil;

• quantification of released CO₂ and other volatile compounds;

• plots of soil concentrations versus time for the test substance and, where appropriate, for major transformation products;

• half-life or DT₅₀, DT₇₅ and DT₉₀ for the test substance and, where appropriate, for major transformation products including confidence limits;

• estimation of abiotic degradation rate under sterile conditions;

• an assessment of transformation kinetics for the test substance and, where appropriate, for major transformation products;

• proposed pathways of transformation, where appropriate;

• discussion and interpretation of results;

• raw data (i.e. sample chromatograms, sample calculations of transformation rates and means used to identify transformation products).