Division 1.9.1 Test conditions

The test-should be performed in the incubation apparatus (see section 1.8.1) with a water sediment volume ratio between 3:1 and 4:1, and a sediment layer of 2.5 cm (± 0.5 cm).¹ A minimum amount of 50 g of sediment (dry weight basis) per incubation vessel is recommended.

The test should be performed in the dark at a constant temperature in the range of 10 to 30 ^oC. A temperature of (20 ± 2)^oC is appropriate. Where appropriate, an additional lower temperature (e.g. 10^oC) may be considered on a case-by-case basis, depending on the information required from the test. Incubation temperature should be monitored and reported.