Division TEST REPORT

The test report must include the following information:

Complete identification of the soil used including:

• geographical reference of the site (latitude, longitude);

• information on the history of the site (i.e. vegetation cover, treatments with crop protection products, treatments with fertilisers, accidental contamination, etc.)

• use pattern (e.g. agricultural soil, forest, etc.);

• depth of sampling (cm);

• sand/silt/clay content (% dry weight);

• pH (in water);

• organic carbon content (% dry weight);

• nitrogen content (% dry weight);

• cation exchange capacity (mmol/kg);

• initial microbial biomass in terms of percentage of the total organic carbon;

• reference of the methods used for the determination of each parameter;

• all information relating to the collection and storage of soil samples;

• details of pre-incubation of soil if any.

Test substance:

• physical nature and, where relevant, physical-chemical properties;

• chemical identification data, where relevant, including structural formula, purity (i.e. for crop protection products the percentage of active ingredient), nitrogen content.

Test conditions:

• details of the amendment of soil with organic substrate;

• number of concentrations of test chemical used and, where appropriate, justification of the selected concentrations;

• details of the application of test substance to soil;

• incubation temperature;

• soil moisture content at the beginning and during the test;

• method of soil incubation used (i.e. as bulk or as a series of individual subsamples);

• number of replicates;

• sampling times.

Results:

• method and equipment used for measurement of respiration rates;

• tabulated data including individual and mean values for quantities of carbon dioxide or oxygen;

• variation between the replicates in treated and control samples;

• explanations of corrections made in the calculations, if relevant;

• the percent variation of glucose-induced respiration rates at each sampling time or, if appropriate, the EC₅₀ with 95 per cent confidence limit, other EC_x (i.e. EC₂₅ or EC₁₀) with confidence intervals, and a graph of the dose-response curve;

• statistical treatment of results, where appropriate;

• all information and observations helpful for the interpretation of the results.