Division (3) Annex V to Directive 2000/54/EC is replaced by the following:

United Kingdom

‘ANNEX V

INDICATIONS CONCERNING CONTAINMENT MEASURES AND CONTAINMENT LEVELS

(Articles 15(3) and 16(1)(a) and (b))

Preliminary note

The measures contained in this Annex shall be applied according to the nature of the activities, the assessment of risk to workers, and the nature of the biological agent concerned.

In the table, “Recommended” means that the measures should in principle be applied, unless the results of the assessment referred to in Article 3(2) indicate otherwise.

HEPA: High efficiency particulate air

Airlock: Entry must be through an airlock which is a chamber isolated from the laboratory. The clean side of the airlock must be separated from the restricted side by changing or showering facilities and preferably by interlocking doors.

A. Containment measures B. Containment levels
2 3 4
Workplace
1. The workplace is to be separated from any other activities in the same building No Recommended Yes
2. The workplace is to be sealable to permit fumigation No Recommended Yes
Facilities
3. Infected material including any animal is to be handled in a safety cabinet or isolation or other suitable containment Where appropriate Yes, where infection is by airborne route Yes
Equipment
4. Input air and extract air to the workplace are to be filtered using (HEPA^f1) or likewise No Yes, on extract air Yes, on input and extract air
5. The workplace is to be maintained at an air pressure negative to atmosphere No Recommended Yes
6. Surfaces impervious to water and easy to clean Yes, for bench and floor Yes, for bench, floor and other surfaces determined by risk assessment Yes, for bench, walls, floor and ceiling
7. Surfaces resistant to acids, alkalis, solvents, disinfectants Recommended Yes Yes
System of work
8. Access is to be restricted to nominated workers only Recommended Yes Yes, via airlock^f2
9. Efficient vector control, for example rodents and insects Recommended Yes Yes
10. Specified disinfection procedures Yes Yes Yes
11. Safe storage of a biological agent Yes Yes Yes, secure storage
12. Personnel should shower before leaving the contained area No Recommended Recommended
Waste
13. Validated inactivation process for the safe disposal of animal carcases Recommended Yes, on or off site Yes, on site
Other measures
14. A laboratory is to contain its own equipment No Recommended Yes
15. An observation window, or, alternative, is to be present, so that occupants can be seen Recommended Recommended Yes

’

HEPA: High efficiency particulate air
Airlock: Entry must be through an airlock which is a chamber isolated from the laboratory. The clean side of the airlock must be separated from the restricted side by changing or showering facilities and preferably by interlocking doors.
A. Containment measures	B. Containment levels
2	3	4
Workplace
1. The workplace is to be separated from any other activities in the same building	No	Recommended	Yes
2. The workplace is to be sealable to permit fumigation	No	Recommended	Yes
Facilities
3. Infected material including any animal is to be handled in a safety cabinet or isolation or other suitable containment	Where appropriate	Yes, where infection is by airborne route	Yes
Equipment
4. Input air and extract air to the workplace are to be filtered using (HEPA^f1) or likewise	No	Yes, on extract air	Yes, on input and extract air
5. The workplace is to be maintained at an air pressure negative to atmosphere	No	Recommended	Yes
6. Surfaces impervious to water and easy to clean	Yes, for bench and floor	Yes, for bench, floor and other surfaces determined by risk assessment	Yes, for bench, walls, floor and ceiling
7. Surfaces resistant to acids, alkalis, solvents, disinfectants	Recommended	Yes	Yes
System of work
8. Access is to be restricted to nominated workers only	Recommended	Yes	Yes, via airlock^f2
9. Efficient vector control, for example rodents and insects	Recommended	Yes	Yes
10. Specified disinfection procedures	Yes	Yes	Yes
11. Safe storage of a biological agent	Yes	Yes	Yes, secure storage
12. Personnel should shower before leaving the contained area	No	Recommended	Recommended
Waste
13. Validated inactivation process for the safe disposal of animal carcases	Recommended	Yes, on or off site	Yes, on site
Other measures
14. A laboratory is to contain its own equipment	No	Recommended	Yes
15. An observation window, or, alternative, is to be present, so that occupants can be seen	Recommended	Recommended	Yes

Annotations

Footnotes

f1: HEPA: High efficiency particulate air
f2: Airlock: Entry must be through an airlock which is a chamber isolated from the laboratory. The clean side of the airlock must be separated from the restricted side by changing or showering facilities and preferably by interlocking doors.