Division 6.2 Operating conditionsF1
United Kingdom
Optimize the temperature programme and the carrier gas flow so that chromatograms similar to Figures 3 to 6 are obtained.
The following parameters were tested and found useful:
These conditions may be changed according to the characteristics of the column and gas chromatograph, so as to obtain chromatograms, which meet the following requirements:
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Alcohol C26 retention time shall be 18 ± 5 minutes.
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Alcohol C22 peak shall be 80 ± 20 % of the full-scale value for olive oil and 40 ± 20 % of the full-scale value for olive-pomace oil.
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The retention time for the β-sitosterol peak should be at 20 ± 5 min.
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The campesterol peak should be: for olive oil (mean content 3 %) 20 ± 5 % of full scale.
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All the present sterols must be separated. In addition to being separated, the peaks must also be completely resolved, i.e. the peak trace should return to the base line before leaving for the next peak. Incomplete resolution is, however, tolerated, provided that the peak at RRT 1,02 (Sitostanol) can be quantified using the perpendicular.