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Division 6.3.3 F1

United Kingdom

Original text. Later amendments are not shown.

Quantitative analysis

The stigmastadienes content is determined according to the formula:

mg/kg of stigmastadienes = A s × M c A c × M o

where: A s

  • area of stigmastadienes peak (if the peak is resolved into two isomers, sum of areas of the two peaks),

    • A c

  • area of internal standard (cholestadiene),

    • M c

  • mass of standard added, in micrograms,

    • M o

  • mass of oil taken, in grams.

    • Detection limit: about 0,01 mg/kg.

    F2Note 10: When stigmastadienes appear in concentrations of more than 4 mg/kg, if quantifying is required, the method of the International Olive Council for determination of sterenes in refined oil must be applied.

    Annotations

    Textual amendments

    F1

    Inserted by Commission Regulation (EC) No 656/95 of 28 March 1995 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis and Council Regulation (EEC) No 2658/87 on the tariff and statistical nomenclature and on the Common Customs Tariff.

    F2

    Inserted by Commission Implementing Regulation (EU) 2019/1604 of 27 September 2019 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis.