Division 4 METHODF1
United Kingdom
The method for the calculation of the theoretical content of triacylglycerols with ECN 42 and of the difference with respect to the HPLC data is essentially made by the coordination of analytical data obtained by means of other methods. It is possible to distinguish three phases: determination of fatty acid composition by capillary gas chromatography, calculation of theoretical composition of triacylglycerols with ECN 42, HPLC determination of ECN 42 triacylglycerols.
- 4.1Apparatus
- 4.2Reagents
- 4.3Sample preparation
- 4.4HPLC analysis
- 4.5Calculation of triacylglycerols composition (moles %) from fatty acid composition data (area %)