Appendix A F1
United Kingdom
Determination of linear gas speed
Inject 1:3 μl of methane (or propane) into the gas chromatograph after adjusting it to the normal operating conditions. Measure the time the gas takes to run through the column from the moment it is injected until the peak emerges (tM).
The linear speed in cm/s is given by L/tM where L is the length of the column, in cm, and tM is the time measured in s.
Annotations
Textual amendments
Notes on Annex XX
Footnotes
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After elution of the sterol esters, the chromatogram should not show any significant peaks (triacylglycerols).