OpenLegislation

Annex III

United Kingdom

Original text. Later amendments are not shown.

1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5.0 ande 40 °C.

1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5.0 and 40 °C.

1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5.0 ande 40 °C.

1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 °C.

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.

1 U is teh amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.

1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 6,5 and 37 °C.

1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from casein substrate per minute at pH 7,5 and 40 °C.

1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.

1 FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5.5 and 37 °C.

1 IU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 4,5 and 30 °C.

1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 7.5 and 30 °C.

1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 7.5 and 30 °C.

1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7.4 and 37 °C.
No.(or EC No)AdditiveChemical formula, descriptionSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsPeriod of authorisation
Units of activity/kg of complete feedingstuff
12

Endo-1,4-beta-glucanase

EC 3.2.1.4

Preparation of endo-1,4-beta-glucanase, endo-1,3 (4)-beta-glucanase and endo-1,4-beta-xylanase produced by Trichoderma viride (FERM BP-4447) having a minimum activity

  • Endo-1,4-beta-glucanase: 8 000 U/gf1

  • Endo-1,3(4)-glucanase: 18 000 U/gf2

  • Endo-1,4-beta-xylanase: 26 000 U/gf3

Turkeys for fattening—Endo-1,4-beta-glucanase 800 U—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • Endo-1,4-beta-glucanase: 800-1 200 U

  • Endo-1,3 (4)-beta-glucanase: 1 800-2 700 U

  • Endo-1,4-beta-xylanase: 2 600-3 900 U.

3.

For use in compound feed rich in non-strach polysaccarides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20 % wheat and 20 % barley.

30.9.2001

Endo-1,3(4)-beta-glucanase

EC 3.2.1.6

Endo-1,3(4)-beta-glucanase: 1 800 U—

Endo-1,4-beta-xilanase

EC 3.2.1.8

Endo-1,4-beta-xylanase: 2 600 U—
17

Endo-1,4-beta-xylanase

EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:

  • Solid form: 6 000 EPU/gf4

  • Liquid form: 6 000 EPU/ml

Turkeys for fattening—750 EPU—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • 1 500-3 000 EPU.

3.

For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 35 % wheat.

30.9.2001
42

Endo-1,4(4)-betaxylanase

EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:

  • Solid form: 4 000 U/gf5

Characteristic of the authorised preparation:

  • endo-1,4-beta-xylanase: 1,99 %

  • wheat: 97,7 %

  • calcium propionate: 0,3 %

  • lecithin: 0,01 %

Pigs for fattening—4 000 U—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • 4 000 U

3.

For use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 60 % wheat.

30.9.2001
49

Endo-1,3(4)-beta-glucanase

EC 3.2.1.6

Preparation of endo-1,3(4)-beta-glucanase producted by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9554) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:

  • endo-1,3(4)-beta-glucanase: 150 U/gf6

  • endo-1,4-beta-xylanase: 1 500 U/gf7

  • alfa-amylase: 500 U/gf8

  • bacillolysin: 800 U/gf9

  • polygalacturonase: 50 U/gf10

Chickens for fattening—Endo-1,3(4)-beta-glucanase: 150 U—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuffs:

  • endo-1,3(4)-beta-glucanase: 150 U

  • endo-1,4-beta-xylanase: 1 500 U

  • alpha-amylase: 800 U.

  • bacillolysin: 800 U

  • polygalacturonase: 50 U

3.

For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and betaglucans), e.g. containing more than 30 % wheat.

30.9.2001

Endo-1,4-beta-xilanasa

EC 3.2.1.8

Endo-1,4-beta-xylanase: 1 500 U—

Alfa-amylase

EC 3.2.1.1

Alfa-amylase: 500 U—

Bacillolysin

EC 3.4.24.28

Bacillolysin 800 U—

Polygalacturonase

C 3.2.1.15

Polygalacturonase 50 U—
Layinghens—endo-1,3(4)-beta-glucanase: 150 U—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuffs:

  • endo-1,3(4)-beta-glucanase: 150 U

  • endo-1,4-beta-xylanase: 1 500 U

  • alpha-amylase: 1 000 U

  • polygalacturonase: 25 U.

3.

For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and betaglucans), e.g. containing more than 30 % wheat.

30.9.2001
endo-1,4-beta-xylanase: 1 500 U—
alpha-amylase: 1 000 U—
bacillolysin: 800 U—
polygalacturonase: 50 U—
50

6-phytase

EC 3.1.3.26

Preparation of 6-phytase produced by Aspergillus oryzae (DSM 11857) having a minimum activity of:

  • Coated form: 2 500 FYT/gf11

  • Liquid form: 5 000 FYT/g

Chickens for fattening—250 FYT—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • 500-1 000 FYT

3.

For use in compound feed containing more than 0,25 % phytin bound phosphorus.

30.9.2001
Laying hens—250 FYT—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • 500-1 000 FYT

3.

For use in compound feed containing more than 0,25 % phytin bound phosphorus.

30.9.2001
Turkeys for fattening—250 FYT—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • 500-1 000 FYT

3.

For use in compound feed containing more than 0,25 % phytin bound phosphorus.

30.9.2001
Piglets2 months500 FYT—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • 500-1 000 FYT

3.

For use in compound feed containing more than 0,25 % phytin bound phosphorus.

30.9.2001
Pigs for fattening—500 FYT—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • 500-1 000 FYT

3.

For use in compound feed containing more than 0,25 % phytin bound phosphorus.

30.9.2001
51

Endo-1,4-betaxylanase

EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG-S 15136) having a minimum activity of:

Chickens for fattening—10 IU—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • 10- IU

3.

For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.

30.9.2001
52

Endo-1,3(4)-beta-glucanase:

EC 3.2.1.6

Preparation of endo-1,3(4)-beta-glucanasa produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), having a minimum activity of:

  • Liquid form:

    • Endo-1,3 (4)-beta-glucanase: 10 000 U/mf13

    • Endo-1,4-beta-glucanase: 120 000 U/mf14

    • Alpha-amylase: 400 U/mlf15

Chickens for fattening—Endo-1,3(4)-beta-glucanase: 1 000 U—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

Recommended dose per kg of complete feedingstuff:

  • endo-1,3(4)-beta-glucanase: 1 000-2 000 U endo-1,4-beta-glucanase: 12 000-24 000 U

3.

For use in compound feed rich in non starch polysaccharides (mainly arabinoxylans and betaglucans) e.g. containing more than 20 % wheat and 15 % sorghum and 5 % maize.

30.9.2001

Endo-1,4-beta-glucanase:

EC 3.2.1.4

Endo-1,4-beta-glucanase: 12 000 U—

Alpha-amylase

EC 3.2.2.1

Alpha-amylase: 40 U—

Annotations

Footnotes

f1

1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5.0 ande 40 °C.

f2

1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5.0 and 40 °C.

f3

1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5.0 ande 40 °C.

f4

1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 °C.

f5

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.

f6

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.

f7

1 U is teh amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.

f8

1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 6,5 and 37 °C.

f9

1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from casein substrate per minute at pH 7,5 and 40 °C.

f10

1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.

f11

1 FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5.5 and 37 °C.

f12

1 IU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 4,5 and 30 °C.

f13

1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 7.5 and 30 °C.

f14

1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 7.5 and 30 °C.

f15

1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7.4 and 37 °C.