Division 5.1 F1
United Kingdom
A high-performance liquid chromatograph capable of functioning in binary gradient mode and equipped with:
- 5.1.1.
A spectrophotometric detector capable of measuring at wavelengths from 260 to 340 nm. It is however preferable to work with a multiple wavelength detector with a diode array or similar, in order to confirm the purity of the peaks.
- 5.1.2.
A spectrofluorimetric detector — excitation wavelength: 354 nm, emission wavelength: 446 nm (for the trace determination of scopoletin; which is also detectable at 313 nm by spectrophotometry).
- 5.1.3.
An injection device capable of introducing 10 or 20 μl (for example) of the test sample.
- 5.1.4.
A high-performance liquid chromatography column, RP C18 type, 5 μm maximum particle size.