Division HPLC conditions
United Kingdom
The following conditions are offered for guidance; other conditions may be used provided that they give equivalent results.
| Liquid chromatographic column (4.5.1): | 250 mm × 4 mm, C18, 5 or 10 μm packing, or equivalent |
| Mobile phase (3.9): | Mixture of methanol (3.3) and water e.g. 980 + 20 (v + v). |
| Flow rate: | 1-2 ml/min. |
| Detector (4.5.2): | UV detector (325 nm) or fluorescence detector (excitation: 325 nm/emission: 475 nm) |