Division 3.8 HPLC mobile phase.

Mix together the following reagents:

• 650 ml acetonitrile (3.3),

• 250 ml water (equivalent to HPLC-grade),

• 50 ml potassium di-hydrogen phosphate solution (3.6),

• 50 ml di-sodium hydrogen phosphate solution (3.7).

Filter through a 0,22 μm filter (4.6) and degas the solution, (e.g. by ultrasonification for 10 minutes).