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Division 5.3.1 Parameters

United Kingdom

Original text. Later amendments are not shown.

The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results.

Liquid chromatographic column (4.2.1)100 mm × 4,6 mm, Hypersil ODS, 3 μm packing, or equivalent
Mobile phase:Eluent A (3.13.1):Aqueous solution of ammonium acetate and tetrabutyl-ammonium hydrogen sulphate
Eluent B (3.13.2):acetonitrile
Eluent C (3.13.3):methanol
Elution mode:

  • linear gradient

  • initial conditions: A + B + C = 60 + 20 + 20 (V + V + V)

  • after 10 min. gradient elution during 30 min. to: A + B + C = 45 + 20 + 35 (V + V + V)

Flush with B during 10 min.

Flow rate:1,5-2 ml/min.
Injection volume:20 μl
Detector wavelength:280 nm.

Check the stability of the chromatographic system, injecting several times the calibration solution (3.10), containing 2 μg/ml, until constant peak heights and retention times are achieved.