Division 5.3.1 Parameters:
United Kingdom
The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results.
| Analytical column (4.3.1) | |
| Mobile Phase (3.4): | water (3.3)-methanol (3.2) mixture, 900 + 100 (V + V) |
| Flow rate: | 1,5-2 ml/min. |
| Detection wavelength: | 380 nm |
| Injection volume: | 20 μl –100 μl |
Check the stability of the chromatographic system, injecting several times the calibration solution (3.5.3) containing 2,5 μg/ml, until constant peak heights and retention times are achieved.