OpenLegislation

Division 3.1 TEST REPORT

United Kingdom

Original text. Later amendments are not shown.

The test report must include the following:

Test substance:

  • physical nature and physicochemical properties, including water solubility limit,

  • chemical identification data (e.g. CAS Number), including purity.

Test species:

  • scientific name, clone (if known) and source.

Test conditions:

  • test procedure used (static, semi-static or flow-through),

  • date of start of the test and its duration,

  • test medium,

  • description of the experimental design: test vessels and covers, solution volumes, number of colonies and fronds per test vessel at the beginning of the test,

  • test concentrations (nominal and measured as appropriate) and number of replicates per concentration,

  • methods of preparation of stock and test solutions including the use of any solvents or dispersants,

  • temperature during the test,

  • light source, light intensity and homogeneity,

  • pH values of the test and control media,

  • test substance concentrations and the method of analysis with appropriate quality assessment data (validation studies, standard deviations or confidence limits of analyses),

  • methods for determination of frond number and other measurement variables, e.g. dry weight, fresh weight or frond area,

  • all deviations from this Testing Method.

Results:

  • raw data: number of fronds and other measurement variables in each test and control vessel at each observation and occasion of analysis,

  • means and standard deviations for each measurement variable,

  • growth curves for each concentration (recommended with log transformed measurement variable, see second paragraph in section 2.2.1),

  • doubling time/growth rate in the control based on the frond number,

  • calculated response variables for each treatment replicate, with mean values and coefficient of variation for replicates,

  • graphical representation of the concentration/effect relationship,

  • estimates of toxic endpoints for response variables e.g. EC50, EC10, EC20, and associated confidence intervals. If calculated, LOEC and/or NOEC and the statistical methods used for their determination,

  • if ANOVA has been used, the size of the effect which can be detected (e.g. the least significant difference),

  • any stimulation of growth found in any treatment,

  • any visual signs of phytotoxicity as well as observations of test solutions,

  • discussion of the results, including any influence on the outcome of the test resulting from deviations from this Testing Method.