Division 4.2.5 Amplification

Amplification cycles are performed in a thermal cycle apparatus.

• Initial denaturation: 2 min at 94 °C

• Amplification: 35 cycles (1 min at 94 °C, 1 min at 50 °C and 1 min at 72 °C)

• Final elongation: 5 min at 72 °C