Division 3.2 Detection method

F1The detection of the relevant Salmonella serotypes must be carried out according to ISO 6579-1 as it has effect for the time being.

As regards the boot swabs samples, dust samples and other faecal material samples referred to in point 3.1, the incubated BPW enrichment broth for future culture may be pooled. To do so, incubate both samples in BPW as referred to in point 3.1.3. Take 1 ml of incubated broth from each sample and mix thoroughly, then take 0,1 ml of the mixture and inoculate the modified semi-solid Rappaport-Vassiliadis (MSRV) plates.

The samples in BPW must not be shaken, swirled or otherwise agitated after incubation as this releases inhibitory particulates and reduces subsequent isolation in MSRV.