Division 5.2 Eluent solution, pH 6,0F1
United Kingdom
Dissolve 1,74 g of dipotassium hydrogen phosphate (K 2 HPO 4 ), 12,37 g of potassium dihydrogen phosphate (KH 2 PO 4 ) and 21,41 g of sodium sulphate (Na 2 SO 4 ) in about 700 ml of water. Adjust, if necessary, to pH 6,0, using a solution of phosphoric acid or potassium hydroxide.
Make up to 1 000 ml with water and homogenise.
Filter the eluent solution, prior to use, through a membrane filter with a 0,45 μm pore diameter.