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Commission Implementing Regulation (EU) 2016/1240 of 18 May 2016 laying down rules for the application of Regulation (EU) No 1308/2013 of the European Parliament and of the Council with regard to public intervention and aid for private storage (Text with EEA relevance)

EU Regulation 2016/1240 · Adopted 18 May 2016 · Extends to United Kingdom

Original text. legislation.gov.uk does not revise this item, so later amendments are not shown here. Changes made to it are listed under each provision when known.

Commission Implementing Regulation (EU) 2016/1240

of 18 May 2016

laying down rules for the application of Regulation (EU) No 1308/2013 of the European Parliament and of the Council with regard to public intervention and aid for private storage

(Text with EEA relevance)

C1C2C3C4

THE EUROPEAN COMMISSION,

Having regard to the Treaty on the Functioning of the European Union,

Having regard to Regulation (EU) No 1308/2013 of the European Parliament and of the Council of 17 December 2013 establishing a common organisation of the markets in agricultural products and repealing Council Regulations (EEC) No 922/72, (EEC) No 234/79, (EC) No 1037/2001 and (EC) No 1234/2007f1, and in particular Article 20(a), (b), (d), (e), (i), (j), (k), (l), (m), (n) and (o), and Article 223(3)(a), (b) and (c) thereof,

Having regard to Regulation (EU) No 1306/2013 of the European Parliament and of the Council of 17 December 2013 on the financing, management and monitoring of the common agricultural policy and repealing Council Regulations (EEC) No 352/78, (EC) No 165/94, (EC) No 2799/98, (EC) No 814/2000, (EC) No 1290/2005 and (EC) No 485/2008f2, and in particular Article 62(2)(a), (b), (c) and (i), and Article 64(7)(a) thereof,

Having regard to Council Regulation (EU) No 1370/2013 of 16 December 2013 determining measures on fixing certain aids and refunds related to the common organisation of the markets in agricultural productsf3, and in particular Article 2(3) and Article 3(4) thereof,

Whereas:

(1)

Regulation (EU) No 1308/2013 replaced Council Regulation (EC) No 1234/2007f4 and lays down new rules regarding public intervention and aid for private storage. It also empowers the Commission to adopt delegated and implementing acts in that respect. In order to ensure the smooth functioning of the public intervention and aid for private storage schemes in the new legal framework, certain rules have to be adopted by means of such acts. Those acts should replace Commission Regulations (EEC) No 3427/87f5, (EEC) No 2351/91f6, (EC) No 720/2008f7, (EC) No 826/2008f8, (EC) No 1130/2009f9 and (EU) No 1272/2009f10. Those Regulations are repealed by Commission Delegated Regulation (EU) 2016/1238f11.

(2)

Article 11 of Regulation (EU) No 1308/2013 provides that public intervention applies in respect of common wheat, durum wheat, barley, maize, paddy rice, fresh or chilled meat of the beef and veal sector, butter and skimmed milk powder in accordance with the conditions set out in that Regulation.

(3)

Article 17 of Regulation (EU) No 1308/2013 provides that aid for private storage may be granted in respect of white sugar, olive oil, flax fibre, fresh or chilled meat of bovine animals aged eight months or more, butter, cheese, skimmed milk powder, pigmeat, and sheepmeat and goatmeat in accordance with the conditions set out in that Regulation.

(4)

Regulation (EU) No 1370/2013 lays down rules concerning public intervention prices, quantitative limitations for buying-in into public intervention and the establishment of the amount of aid for private storage.

(5)

In order to simplify and improve the effectiveness of the management and control mechanisms related to the public intervention and aid for private storage schemes, common rules for all the products listed in Articles 11 and 17 of Regulation (EU) No 1308/2013 should respectively be laid down.

(6)

In accordance with Article 13(1) of Regulation (EU) No 1308/2013, the Commission may decide that buying in of durum wheat, barley, maize and paddy rice is to take place if the market situation so requires. Public intervention may also be opened for beef if the average market price is over a representative period below 85 % of the reference threshold set out in Article 7(1) of Regulation (EU) No 1308/2013. In such cases buying-in takes place by means of tendering procedures.

(7)

In accordance with Article 4(1) of Regulation (EU) No 1370/2013, the Commission may establish the amount of aid for private storage for the products listed in Article 17 of Regulation (EU) No 1308/2013 either by a tendering procedure or by fixing the aid in advance.

(8)

To make the public intervention and aid for private storage schemes work efficiently, the operators should use the method made available by the paying agency with regard to the procedure for submitting offers, tenders and applications.

(9)

In order to ensure proper management of the schemes, rules should be established on intervention at a fixed price, the tendering procedures for buying-in into intervention, sales from intervention or establishing the maximum amount of aid for private storage, and the aid for private storage fixed in advance as well as the submission and admissibility of tenders, offers and applications.

(10)

In order to improve the efficiency of the way public intervention operates, by discontinuing the use of small storage facilities which may be spread throughout a region, a minimum capacity of storage should be fixed for the intervention storage places, but should not be applicable in case where a storage place has ready access to a river, sea or railway connection.

(11)

Since products covered by the public intervention and aid for private storage schemes have a different nature as to production or harvest time and storage requirements, specific conditions concerning their eligibility should be provided for.

(12)

For a sound management of the public intervention and aid for private storage schemes, it is necessary to fix a minimum quantity below which the paying agency cannot accept an offer or a tender, both for buying-in and sales, or decide whether to fix or not the maximum amount of aid for private storage. However, if the conditions and practices of the wholesale trade or environmental rules in force in a Member State justify the application of minimum quantities larger than those laid down in this Regulation, the paying agency concerned should be entitled to require such larger minimum quantities for buying-in at fixed price.

(13)

In order to guarantee the seriousness of the offer or tender for intervention and to ensure that the measure will have its desired effect on the market, both in the case of intervention at fixed price and in the case of a tendering procedure, the level of the security should be established.

(14)

To ensure an effective management of the public intervention scheme, notifications on submitted offers and tenders should be provided for between Member States and the Commission. Measures should be introduced to respect the quantitative limitations fixed in Article 3 of Regulation (EU) No 1370/2013.

(15)

On the basis of the offers and tenders received a maximum buying-in price or a maximum amount of aid for private storage may be fixed. However, situations might arise on the market in which economic or other aspects make it necessary not to fix such a price or aid and to reject all the tenders received.

(16)

In order to guarantee a clear and effective functioning of the public intervention scheme, it is necessary to lay down the general rules concerning the issuance of the delivery order and the delivery of the products to the storage place designated by the paying agency. In addition, given the specificities of the sectors of cereals and rice as well as beef and butter and skimmed milk powder, it is necessary to lay down specific rules for those sectors.

(17)

With a view to the proper management of intervention stocks in storage and given the specificities of the sectors of cereals and rice, the obligations of the Member States should be specified as regards the maximum distance to the place of storage and the costs to be borne when this distance is exceeded.

(18)

In order to apply the common rules of Commission Delegated Regulation (EU) No 907/2014f12, it is appropriate to provide that the checks on products during storage have to be carried out as laid down in Article 3 of that Regulation. A takeover record should be issued on the basis of those checks and analyses.

(19)

In order to ensure good quality of products stored under public intervention, in case the products do not fulfil the applicable eligibility requirements, it should be provided for an obligation of the operator to take back the products and bear all the costs during the period the products have been stored in the storage places.

(20)

Where deboning is required for beef, it is necessary to lay down special rules for that sector to complement the general ones.

(21)

Rules should be established for payments, subject to price adjustment related to the quality of the product or to the location of the storage place. In order to give the operators the time to adapt to the new public intervention scheme, some conditions relating to price adjustment with regard to cereals should be applicable only from the start of the 2017/18 marketing year.

(22)

In order to give the necessary information on the characteristics of the products and on the site where they are stored, notices of invitation to tender should be drawn up and published by the paying agency holding intervention stocks available for sale. To this end, it should be provided for that a reasonable time period should elapse between the date of such publication and the first closing date for the submission of tenders.

(23)

On the basis of the tenders and of the situation on the Union market, the Commission should decide to fix or not a minimum intervention selling price. According to that decision, the paying agencies will accept or reject the tenders on products available for sale. Specific rules should be adopted for the allocation of beef, butter and skimmed milk powder.

(24)

In order to facilitate the sale of small quantities remaining in storage places in a Member State and to assure a sound management of the system, it is appropriate to provide that the paying agency, under its own responsibility, is entitled to open the tendering procedure for the resale of such quantities of intervention products, by applying mutatis mutandis the rules laid down in respect of tendering procedures opened by the Union, with a view to ensure equal access for all parties concerned. For the same reasons the paying agency should be authorised to put up for direct sale quantities which after checking by visual examination in the context of the annual stocktaking or during the inspection after taking into intervention may no longer be repackaged or are deteriorated.

(25)

To ensure that the aid for private storage scheme can be monitored properly, the information needed to conclude the storage contract should be specified as well as the obligations of the contracting parties and the conditions for placing into storage, in particular those enabling the competent authority responsible for checking storage operations to make an effective inspection of the storage conditions. The rules with regard to the contractual storage period should also be defined.

(26)

In order to ensure an efficient functioning of the aid for private storage scheme, it is necessary to lay down the general rules concerning the removal of the products from storage and the payment of aid for private storage. For butter and skimmed milk powder, given the specific nature of these products, special rules are necessary to complement the general ones.

(27)

When the amount of aid for private storage is fixed in advance, it is appropriate to provide for a reflection period in order to allow the market situation to be assessed before decisions on applications for aid are notified. Furthermore, where appropriate, provisions should be made for special measures applying in particular to pending applications to prevent excessive or speculative use of the private storage scheme. Such measures require swift action and the Commission should be empowered to act without applying the procedure referred to in Article 229(2) or (3) of Regulation (EU) No 1308/2013 and to take all necessary measures without delay.

(28)

To protect the Union's financial interests, adequate control measures should be adopted to combat irregularities and fraud. These control measures should involve full administrative checking supplemented by on-the-spot checks. The scope, content, timing and reporting of such control measures should be specified so as to ensure an equitable and uniform approach between Member States.

(29)

Amounts unduly paid should be recovered in accordance with Commission Implementing Regulation (EU) No 908/2014f13.

(30)

For an effective management of the public intervention and aid for private storage schemes, it is necessary to provide that the Member States inform the Commission periodically of the situation of stocks, of the products entering and leaving storage places and of the situation regarding prices and production for the products listed in Articles 11 and 17 of Regulation (EU) No 1308/2013.

(31)

The measures provided for in this Regulation are in accordance with the opinion of the Committee for the Common Organisation of the Agricultural Markets,

HAS ADOPTED THIS REGULATION:

Annotations to the whole text

Modifications

C1

Regulation: power to modify conferred (E.) (11.11.2020 for specified purposes, 11.1.2021 in so far as not already in force) by Agriculture Act 2020 (c. 21), ss. 22, 57(1)(b)(c)(6)

C2

Regulation: power to modify conferred (N.I.) (11.11.2020 for specified purposes) by Agriculture Act 2020 (c. 21), s. 57(1)(b)(c)(6), Sch. 6 para. 9

C3

Regulations applied (with modifications) (S.) (8.11.2021) by The Private Storage Aid Scheme (Pigmeat) (Scotland) Regulations 2021 (S.S.I. 2021/398), regs. 1(1), 3(7), 6 (with reg. 5)

C4

Regulation: power to modify conferred (W.) (17.10.2023) by Agriculture (Wales) Act 2023 (asc 4), ss. 23, 56(4); S.I. 2023/1092, art. 2(a)

Footnotes

f1

OJ L 347, 20.12.2013, p. 671.

f2

OJ L 347, 20.12.2013, p. 549.

f3

OJ L 346, 20.12.2013, p. 12.

f4

Council Regulation (EC) No 1234/2007 of 22 October 2007 establishing a common organisation of agricultural markets and on specific provisions for certain agricultural products (Single CMO Regulation) (OJ L 299, 16.11.2007, p. 1).

f5

Commission Regulation (EEC) No 3427/87 of 16 November 1987 laying down detailed rules for intervention on the market in rice (OJ L 326, 17.11.1987, p. 25).

f6

Commission Regulation (EEC) No 2351/91 of 30 July 1991 laying down detailed rules applicable on the purchase of rice held by an intervention agency for the supply of food aid (OJ L 214, 2.8.1991, p. 51).

f7

Commission Regulation (EC) No 720/2008 of 25 July 2008 laying down common detailed rules for the application of Council Regulation (EC) No 1234/2007 as regards the storage and movement of products bought in by a paying agency or an intervention agency (OJ L 198, 26.7.2008, p. 17).

f8

Commission Regulation (EC) No 826/2008 of 20 August 2008 laying down common rules for the granting of private storage aid for certain agricultural products (OJ L 223, 21.8.2008, p. 3).

f9

Commission Regulation (EC) No 1130/2009 of 24 November 2009 laying down common detailed rules for verifying the use and/or destination of products from intervention (OJ L 310, 25.11.2009, p. 5).

f10

Commission Regulation (EU) No 1272/2009 of 11 December 2009 laying down common detailed rules for the implementation of Council Regulation (EC) No 1234/2007 as regards buying-in and selling of agricultural products under public intervention (OJ L 349, 29.12.2009, p. 1).

f11

Commission Delegated Regulation (EU) 2016/1238 of 18 May 2016 supplementing Regulation (EU) No 1308/2013 of the European Parliament and of the Council with regard to public intervention and aid for private storage (see page 15 of this Official Journal).

f12

Commission Delegated Regulation (EU) No 907/2014 of 11 March 2014 supplementing Regulation (EU) No 1306/2013 of the European Parliament and of the Council with regard to paying agencies and other bodies, financial management, clearance of accounts, securities and use of euro (OJ L 255, 28.8.2014, p. 18).

f13

Commission Implementing Regulation (EU) No 908/2014 of 6 August 2014 laying down rules for the application of Regulation (EU) No 1306/2013 of the European Parliament and of the Council with regard to paying agencies and other bodies, financial management, clearance of accounts, rules on checks, securities and transparency (OJ L 255, 28.8.2014, p. 59).

Contents

  1. Title I: GENERAL PROVISIONS
  2. Chapter I: Introductory provision
  3. 1Scope
  4. 1ADefinitions
  5. Chapter II: General common rules
  6. 2Submission and admissibility of offers, tenders and applications
  7. Title II: PUBLIC INTERVENTION
  8. Chapter I: Specific rules related to public intervention
  9. 3Intervention storage places
  10. 4Establishing the eligibility of products
  11. Chapter II: Buying-in of products into intervention
  12. General provisions
  13. 5Minimum quantities of products offered or tendered
  14. 6Level of the security for buying-in of products
  15. 7Submission and admissibility of offers and tenders
  16. 8Verification of offers and tenders by the paying agency
  17. 9Notifications of offers and tenders to the Commission Repealed
  18. Buying-in at fixed price
  19. 10Submission of offers for buying-in of common wheat, butter and skimmed milk powder at fixed price
  20. 11Measures for respecting the quantitative limitations
  21. Buying-in via a tendering procedure
  22. 12Tendering procedure
  23. 13Submission and admissibility of tenders
  24. 14Decisions on the buying-in price
  25. 15Individual decisions on tenders
  26. 16Limitation of buying-in for beef
  27. Deliveries and transport costs
  28. 17Delivery order
  29. 18Specific provisions for the delivery of cereals and rice
  30. 19Transport costs for cereals and rice
  31. 20Specific provisions for the delivery of beef
  32. 21Specific provisions for the packing, delivery and storage of butter and skimmed milk powder
  33. 22Delivery
  34. Control and takeover
  35. 23The takeover record
  36. 24Obligations of the operator
  37. 25Deboning requirement for beef
  38. Price adjustments and payments
  39. 26Price adjustments for cereals and rice
  40. 27Payments
  41. Chapter III: Sale of products from intervention
  42. 28Opening of the tendering procedure
  43. 29Notice of invitation to tender and arrangements related to the invitation to tender
  44. 30Submission and admissibility of tenders
  45. 31Notification of tenders to the Commission Repealed
  46. 32Decisions on the selling price
  47. 33Individual decisions on tenders
  48. 34Specific rules for the allocation of beef, butter and skimmed milk powder
  49. 35Payments
  50. 36Sales by the paying agency
  51. 37Removal order
  52. 38Removal of butter and skimmed milk powder
  53. Chapter IV: Disposal of products from intervention for the scheme for food distribution to the most deprived in the Union Repealed
  54. 38aMaking products from intervention available for the scheme Repealed
  55. 38bSale of intervention products made available for the scheme Repealed
  56. Title III: AID FOR PRIVATE STORAGE
  57. Chapter I: Specific rules for aid for private storage
  58. General provisions
  59. 39Opening of tendering procedures and fixing aid in advance
  60. 40Submission and admissibility of tenders and applications for aid for private storage
  61. 41Verification of tenders and applications by the paying agency
  62. 42Notification of tenders and applications to the Commission Repealed
  63. Fixing the amount of aid for private storage via a tendering procedure
  64. 43Decisions on the maximum amount of aid for private storage
  65. 44Individual decisions on tenders
  66. Fixing the amount of aid for private storage in advance
  67. 45Decisions on applications for aid for private storage fixed in advance
  68. Placing of products into private storage
  69. 46Information related to the place of private storage for products not yet in storage
  70. 47Placing into storage of products not yet in storage
  71. Chapter II: Storage contracts
  72. Conclusion of contracts
  73. 48Contractual storage period
  74. 49Conclusion of contracts
  75. 50Notification of conclusion of contracts
  76. Elements of the contract and obligations of the operator
  77. 51Elements of the contract
  78. 52Obligations of the operator
  79. Chapter III: Removal of products and payment of aid for private storage
  80. Removal of products from storage
  81. 53Removal from storage
  82. Payment
  83. 54Application for payment of aid for private storage
  84. 55Payment of aid for private storage
  85. Title IV: CHECKS AND PENALTIES
  86. Chapter I: Checks
  87. 56General provisions on checks relating to public intervention and aid for private storage
  88. 57Specific provisions on checks relating to public intervention
  89. 58Specific provisions on checks relating to public intervention for cereals and rice
  90. 59Specific provisions for the takeover in storage place of the storekeeper for cereals and rice
  91. 60Specific provisions on checks relating to aid for private storage
  92. 60aSpecific provision on checks relating to public intervention and aid for private storage for milk and milk products
  93. 61Reporting of checks
  94. Chapter II: Penalties and administrative measures
  95. 62Penalties and administrative measures in relation to aid for private storage
  96. Title V: NOTIFICATIONS AND FINAL PROVISIONS
  97. Chapter I: Notifications
  98. General provisions on notifications
  99. 63Method of notification Repealed
  100. 64Notifications concerning the paying agency
  101. Notifications as regards public intervention
  102. 65Notifications of information on intervention stocks
  103. Notifications as regards aid for private storage
  104. 66Notification of information on private storage Repealed
  105. Chapter II: Final provisions
  106. 67Entry into force and application
  107. Signature
  108. Annex I: CEREALS
  109. A1Division A1
  110. A2Division A2
  111. A3Division A3
  113. DEFINITION OF MATTER OTHER THAN BASIC CEREALS OF UNIMPAIRED QUALITY
  114. 1.1Broken grains
  115. Grain impurities
  116. (a)Shrivelled grains
  117. (b)Other cereals
  118. (c)Grains damaged by pests
  119. (d)Grains in which the germ is discoloured
  120. (e)Grains overheated during drying
  121. (f)Mottled grains
  122. 1.3Sprouted grains
  123. Miscellaneous impurities
  124. (a)Extraneous seeds
  125. (b)Damaged grains
  126. (c)Extraneous matter
  127. Husks (cob fragments in the case of maize)
  128. Ergots
  129. (f)Decayed grains
  130. Impurities of animal origin.
  131. Live pests
  132. 1.6Mitadiné grains
  133. SPECIFIC FACTORS TO TAKE INTO CONSIDERATION FOR EACH TYPE OF CEREAL FOR THE DEFINITION OF IMPURITIES
  134. 2.1Durum wheat
  135. 2.2Common wheat
  136. 2.3Barley
  137. 2.4Maize
  139. Methods used for determining the quality of cereals offered or tendered for, or placed in, intervention
  141. Method for determining the non-stickiness and machinability of the dough obtained from common wheat
  142. 1Title
  143. 2Scope
  144. 3Principle
  145. Ingredients
  146. 4.1Yeast
  147. Tap water
  148. 4.3Sugar-salt-ascorbic acid solution
  149. 4.4Sugar solution
  150. 4.5Enzyme active malt flour
  151. Equipment and apparatus
  152. 5.1Baking room
  153. 5.2Refrigerator
  154. 5.3Balance
  155. 5.4Balance
  156. 5.5Analytical balance
  157. 5.6Mixer
  158. 5.7Proving cabinet
  159. 5.8Open plastic boxes
  160. 5.9Square plastic sheets
  161. 5.10Moulder
  162. 6Sampling
  163. Procedure
  164. 7.1Determination of water uptake
  165. 7.2Determination of malt flour addition
  166. 7.3Reactivation of active dry yeast
  167. 7.4Temperature adjustment of the flour and the dough liquid
  168. 7.5Dough composition
  169. 7.6Mixing
  170. 7.7Dividing and rounding
  171. 7.8Moulding
  172. 8Test report
  173. General remarks
  174. 9.1The formula for the calculation of the quantity of dough liquid is based on the following considerations:
  175. 9.2The method is not directly applicable to wheat. The procedure to be followed for characterising the baking properties of wheat is as follows:
  176. Division
  178. Methodology of sampling and analyses for cereals
  179. 1Division 1
  180. 2Division 2
  181. 3Division 3
  183. Price increases and reductions
  185. Calculation of prices increases and reductions
  186. Annex II: RICE
  187. A1Division A1
  188. A2Division A2
  189. A3Division A3
  190. Methodology of sampling and analyses for paddy rice
  191. 1With a view to verifying the quality requirements as laid down in Part I of Annex II to Delegated Regulation (EU) 2016/1238, samples shall be taken by the paying agency in the presence of the operator or his/her duly authorised agent.
  192. 2Representative samples shall be taken of each part-delivery (by lorry, barge, railway wagon) under the conditions laid down in point 1.
  193. 3Division 3
  194. 4In cases of dispute, the paying agency shall have the necessary tests on the paddy rice in question carried out again, the cost being met by the losing party.
  195. PART IIPrices increases and reductions
  196. 1Division 1
  197. 2Division 2
  198. Annex III: BEEF
  199. A1Division A1
  200. A2Division A2
  201. 3ADivision 3A
  202. Conditions and controls for taking over
  203. 1Division 1
  204. 2Division 2
  205. 3Division 3
  206. PART IIConversion coefficients
  207. Deboning
  208. General conditions governing deboning
  209. 1Division 1
  210. 2Division 2
  211. 3Division 3
  212. Contracts and specifications
  213. 1Division 1
  214. 2The specifications of the paying agencies shall lay down the requirements to be met by cutting plants, shall specify the plant and equipment required and shall lay down detailed conditions covering deboning operations.
  215. IIIControl and monitoring of deboning operations
  216. IVStorage of cuts
  217. VCosts of deboning operations
  218. VITime limits for deboning operations
  219. Checks and rejection of products
  220. 1Division 1
  221. 2Division 2
  222. Specifications for intervention deboning
  223. HINDQUARTER CUTS
  224. Description of cuts
  225. 1.1.1Intervention shank (code INT 11)
  226. 1.1.2Intervention thick flank (code INT 12)
  227. 1.1.3Intervention topside (code INT 13)
  228. 1.1.4Intervention silverside (code INT 14)
  229. 1.1.5Intervention fillet (code INT 15)
  230. 1.1.6Intervention rump (code INT 16)
  231. 1.1.7Intervention striploin (code INT 17)
  232. 1.1.8Intervention flank (code INT 18)
  233. 1.1.9Intervention fore-rib (five bone) (code INT 19)
  234. FOREQUARTER CUTS
  235. Description of cuts
  236. 2.1.1Intervention shin (code INT 21)
  237. 2.1.2Intervention shoulder (code INT 22)
  238. 2.1.3Intervention brisket (code INT 23)
  239. 2.1.4Intervention forequarter (code INT 24)
  240. PART VIndividual prices of rejected intervention cuts
  241. Annex IV: BUTTER
  242. A1Division A1
  243. A2Division A2
  244. A3Division A3
  245. Sampling for chemical and microbiological analysis and sensory evaluation
  246. 1Chemical and microbiological analysis
  247. 2Sensory evaluation
  248. Guidelines to be followed where samples show defects
  249. (a)chemical and microbiological analysis:
  250. (b)Division (b)
  251. (c)Division (c)
  252. PART IAMethods of analysis of unsalted butter for public intervention
  253. Scoring table
  254. Delivery and packaging of butter
  255. 1Division 1
  256. 2The packing shall show at least the following particulars, where appropriate in code:
  257. 3Division 3
  258. Annex V: SKIMMED MILK POWDER
  259. A1Division A1
  260. A2Division A2
  261. Sampling and analysis of skimmed-milk powder offered for intervention
  262. 1Division 1
  263. 2Number of packages to be selected for taking samples for analysis:
  264. 3Division 3
  265. 4Division 4
  266. 5Division 5
  267. 6Where samples show defects:
  268. PART IAMethods of analysis of skimmed milk powder for public intervention
  269. Appendix I
  270. SKIMMED MILK POWDER: QUANTITATIVE DETERMINATION OF PHOSPHATIDYLSERINE AND PHOSPHATIDYLETHANOLAMINE
  271. Method: reversed-phase HPLC
  272. 1PURPOSE AND FIELD OF APPLICATION
  273. 2DEFINITION
  274. 3PRINCIPLE OF THE METHOD
  275. 4REAGENTS
  276. Standard material: PEDP, at least 99 % pure
  277. Division
  278. Reagents for standard sample and test sample preparation
  279. 4.2.1Division 4.2.1
  280. 4.2.2Division 4.2.2
  281. 4.2.3Division 4.2.3
  282. Reagents for o-phthaldialdehyde derivatisation
  283. 4.3.1Division 4.3.1
  284. 4.3.2Division 4.3.2
  285. 4.3.3Division 4.3.3
  286. 4.3.4Division 4.3.4
  287. HPLC elution solvents
  288. 4.4.1Division 4.4.1
  289. 4.4.2Division 4.4.2
  290. 4.4.3Division 4.4.3
  291. 4.4.4Division 4.4.4
  292. 4.4.5Division 4.4.5
  293. 4.4.6Division 4.4.6
  294. 4.4.7Division 4.4.7
  295. APPARATUS
  296. 5.1Division 5.1
  297. 5.2Division 5.2
  298. 5.3Division 5.3
  299. 5.4Division 5.4
  300. 5.5Division 5.5
  301. 5.6Division 5.6
  302. 5.7Division 5.7
  303. 5.8Division 5.8
  304. 5.9Division 5.9
  305. 5.10Division 5.10
  306. 5.11Division 5.11
  307. 5.12Division 5.12
  308. HPLC equipment
  309. 5.13.1Division 5.13.1
  310. 5.13.2Division 5.13.2
  311. 5.13.3Division 5.13.3
  312. 5.13.4Division 5.13.4
  313. 5.13.5Division 5.13.5
  314. 5.13.6Division 5.13.6
  315. 6SAMPLING
  316. PROCEDURE
  317. Preparation of the internal standard solution
  318. 7.1.1Division 7.1.1
  319. 7.1.2Division 7.1.2
  320. Preparation of the test sample solution
  321. 7.2.1Division 7.2.1
  322. 7.2.2Division 7.2.2
  323. 7.2.3Division 7.2.3
  324. Division
  325. Preparation of the external standard solution
  326. 7.3.1Division 7.3.1
  327. 7.3.2Division 7.3.2
  328. Division
  329. 7.4Preparation of the derivatising reagent
  330. Division
  331. Determination by HPLC
  332. 7.5.1Elution solvents (4.4)
  333. 7.5.2Suggested eluting gradient:
  334. Division
  335. 7.5.3Division 7.5.3
  336. 7.5.4Column equilibration
  337. Division
  338. 7.5.5Division 7.5.5
  339. 7.5.6Division 7.5.6
  340. Division
  341. Integration mode
  342. 7.6.1PEDP peak
  343. 7.6.2Tryptamine peak
  344. 7.6.3PS and PE peaks groups
  345. 8CALCULATION AND EXPRESSION OF RESULTS
  346. ACCURACY OF THE METHOD
  347. Division
  348. 9.1Repeatability
  349. 9.2Reproducibility
  350. REFERENCES
  351. 10.1Division 10.1
  352. Figure 1HPLC pattern of OPA-derivatives of phosphatidylserine (PS) and phosphatidylethanolamine (PE) in methanol extract of reconstituted skim-milk powder. Integration mode for the peaks of PS, PE and tryptamine (internal standard) is reported
  353. Appendix II
  354. DETECTION OF RENNET WHEY IN SKIMMED MILK POWDER FOR PUBLIC STORAGE BY DETERMINATION OF CASEINOMACROPEPTIDES HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
  355. 1SCOPE AND FIELD OF APPLICATION
  356. 2REFERENCE
  357. 3DEFINITION
  358. 4PRINCIPLE
  359. 5REAGENTS
  360. 5.1Trichloroacetic acid solution
  361. 5.2Eluent solution, pH 6,0
  362. Division
  363. 5.3Flushing solvent
  364. Division
  365. Standard samples
  366. 5.4.1Division 5.4.1
  367. 5.4.2Division 5.4.2
  368. APPARATUS
  369. 6.1Division 6.1
  370. 6.2Division 6.2
  371. 6.3Division 6.3
  372. 6.4Division 6.4
  373. 6.5Division 6.5
  374. 6.6Division 6.6
  375. 6.7Division 6.7
  376. 6.8Division 6.8
  377. 6.9Division 6.9
  378. 6.10Division 6.10
  379. 6.11Division 6.11
  380. SAMPLING
  381. 7.1Division 7.1
  382. 7.2Division 7.2
  383. PROCEDURE
  384. 8.1Preparation of the test sample
  385. 8.2Test portion
  386. Removal of fat and proteins
  387. 8.3.1Division 8.3.1
  388. 8.3.2Division 8.3.2
  389. 8.3.3Division 8.3.3
  390. Chromatographic determination
  391. 8.4.1Division 8.4.1
  392. Division
  393. Division
  394. 8.4.2Division 8.4.2
  395. Calibration
  396. 8.5.1Division 8.5.1
  397. 8.5.2Division 8.5.2
  398. 8.5.3Division 8.5.3
  399. EXPRESSION OF RESULTS
  400. Method of calculation and formulae
  401. 9.1.1Calculation of the response factors R:
  402. 9.1.2Calculation of the relative area of the peaks in the sample [E]
  403. 9.1.3Calculation of the relative retention time of peak III in sample [E]:
  404. 9.1.4Experiments have shown that there is a linear relation between the relative retention time of peak III, i.e. RRT III [E] and the percentage of whey powder added up to 10 %
  405. 9.2Calculation of the percentage of rennet whey powder in the sample:
  406. Accuracy of the procedure
  407. 9.3.1Repeatability
  408. 9.3.2Reproducibility
  409. Interpretation
  410. 9.4.1Division 9.4.1
  411. 9.4.2Division 9.4.2
  412. 9.4.3Division 9.4.3
  413. 9.4.3.1The data obtained after analysis of samples of unadulterated skimmed milk powders with a high total protein content have been assembled in graphs 1 and 2.
  414. 9.4.3.2Division 9.4.3.2
  415. Appendix III
  416. DETERMINING RENNET WHEY SOLIDS IN SKIMMED MILK POWDER
  417. 1Division 1
  418. 2Division 2
  419. 3DEFINITION
  420. 4PRINCIPLE
  421. 5REAGENTS
  422. 5.1Trichloroacetic acid solution
  423. 5.2Eluents A and B
  424. 5.3Conservation of the column
  425. Standard samples
  426. 5.4.1Division 5.4.1
  427. 5.4.2Division 5.4.2
  428. 5.4.3Division 5.4.3
  429. APPARATUS
  430. 6.1Division 6.1
  431. 6.2Division 6.2
  432. 6.3Division 6.3
  433. 6.4Division 6.4
  434. 6.5Division 6.5
  435. 6.6Division 6.6
  436. 6.7Division 6.7
  437. 6.8Division 6.8
  438. 6.9Division 6.9
  439. 6.10Division 6.10
  440. 6.11Division 6.11
  441. SAMPLING
  442. 7.1Division 7.1
  443. 7.2Division 7.2
  444. PROCEDURE
  445. 8.1Preparation of the test sample
  446. 8.2Test portion
  447. Division
  448. Removal of fat and proteins
  449. 8.3.1Division 8.3.1
  450. 8.3.2Division 8.3.2
  451. 8.3.3Division 8.3.3
  452. Chromatographic determination
  453. 8.4.1Division 8.4.1
  454. 8.4.2Division 8.4.2
  455. 8.4.3Take solutions of the test samples
  456. Division
  457. Division
  458. Division
  459. 8.4.4The results of the chromatographic analysis of the test sample (E) are obtained in the form of a chromatogram in which the CMP A peak is identified by its retention time of about 26 minutes
  460. Division
  461. Calibration
  462. 8.5.1Division 8.5.1
  463. Division
  464. 8.5.2Division 8.5.2
  465. 8.5.3Division 8.5.3
  466. EXPRESSION OF RESULTS
  467. Method of calculation and formulae
  468. 9.1.1Calculation of the response factor R:
  469. 9.2Calculation of the percentage of rennet whey powder in the sample
  470. Accuracy of the procedure
  471. 9.3.1Repeatability
  472. 9.3.2Reproducibility
  473. 9.3.3Linearity
  474. 9.4Interpretation
  475. Figure 1Ni—4.6 standard
  476. (*)Division (*)
  477. Delivery and packaging of skimmed milk powder
  478. 1Skimmed milk powder shall be packed in new, clean, dry and intact bags meeting the following requirements:
  479. 2The bags shall show the following particulars, where appropriate in code:
  480. 3Division 3
  481. Annex VIMethods of analysis of butter under private storage
  482. Annex VIIMethods of analysis of skimmed milk powder under private storage
  483. Annex VIIIMethods of analysis of cheeses under private storage
  484. 1The method of analysis laid down in the Appendix shall be used to ensure that cheese made exclusively from ewe's milk, goat's milk or buffalo milk or from a mixture of ewe's milk, goat's milk and buffalo milk does not contain cow's milk casein.
  485. 2Methods for detecting cow's milk casein in cheeses referred to in paragraph 1 may be used provided that:
  486. Attachment
  487. METHOD FOR THE DETECTION OF COW'S MILK AND CASEINATE IN CHEESES FROM EWE'S MILK, GOAT'S MILK OR BUFFALO MILK OR MIXTURES OF EWE'S MILK, GOAT'S MILK AND BUFFALO MILK
  488. 1SCOPE
  489. 2FIELD OF APPLICATION
  490. PRINCIPLE OF THE METHOD
  491. 3.1Division 3.1
  492. 3.2Division 3.2
  493. 3.3Division 3.3
  494. 3.4Division 3.4
  495. 4REAGENTS
  496. Division
  497. Isoelectric focusing
  498. Reagents for production of the urea containing polyacrylamide gels
  499. 4.1.1Stock gel solution
  500. Division
  501. 4.1.2Gel solution
  502. Division
  503. Catalyst solutions
  504. 4.1.3.1Division 4.1.3.1
  505. 4.1.3.2Division 4.1.3.2
  506. Division
  507. 4.2Contact fluid
  508. 4.3Anode solution
  509. 4.4Cathode solution
  510. Sample preparation
  511. Reagents for protein isolation
  512. 4.5.1Division 4.5.1
  513. 4.5.2Division 4.5.2
  514. 4.5.3Division 4.5.3
  515. 4.6Protein dissolving buffer
  516. Division
  517. Reagents for plasmin cleavage of caseins
  518. 4.7.1Ammonium carbonate buffer
  519. 4.7.2Division 4.7.2
  520. 4.7.3ε-Aminocaproic acid solution for enzyme inhibition
  521. Standards
  522. 4.8.1Division 4.8.1
  523. 4.8.2Preparation of laboratory interim-standards of buffalo's renneted milk containing 0 % and 1 % of cow's milk
  524. Division
  525. Reagents for protein staining
  526. 4.9Fixative
  527. 4.10Destaining solution
  528. Division
  529. Staining solutions
  530. 4.11.1Staining solution (stock solution 1)
  531. 4.11.2Staining solution (stock solution 2)
  532. 4.11.3Staining solution (working solution)
  533. Division
  534. EQUIPMENT
  535. 5.1Division 5.1
  536. 5.2Division 5.2
  537. 5.3Division 5.3
  538. 5.4Division 5.4
  539. 5.5Division 5.5
  540. 5.6Division 5.6
  541. 5.7Division 5.7
  542. 5.8Division 5.8
  543. 5.9Division 5.9
  544. 5.10Division 5.10
  545. 5.12Division 5.12
  546. 5.13Division 5.13
  547. 5.14Division 5.14
  548. 5.15Division 5.15
  549. 5.16Division 5.16
  550. 5.17Division 5.17
  551. 5.18Division 5.18
  552. 5.19Division 5.19
  553. PROCEDURE
  554. Sample preparation
  555. 6.1.1Isolation of caseins
  556. Division
  557. 6.1.2Plasmin cleavage of β-caseins to intensify γ-caseins
  558. Division
  559. 6.2Preparation of the urea containing polyacrylamide gels
  560. Division
  561. 6.3lsoelectric focusing
  562. Conditions for isoelectric focusing:
  563. 6.3.1Gel size 265 × 125 × 0,25 mm
  564. Division
  565. 6.3.2Example of a voltage programme for an automatic electrophoresis device (2 gels of 5,0 × 4,5 cm), electrodes without strips applied directly to the gel
  566. Protein staining
  567. 6.4.1Protein fixation
  568. 6.4.2Washing and staining the gel plate
  569. 6.4.3Destaining the gel plate
  570. Division
  571. Division
  572. 7EVALUATION
  573. 7.1Visual estimation
  574. 7.2Densitometric estimation
  575. Division
  576. 8REFERENCES
  577. Figure 1Schematic drawing of the covering sheet
  578. Attachment
  579. Attachment
  580. (*)Division (*)
  581. (**)Division (**)