Division 6.11 F1

HPLC equipment, consisting of:

1. 6.11.1.

   Pump

2. 6.11.2.

   Injector, hand or automatic, with a 15 to 30 μl capacity

3. 6.11.3.

   Two TSK 2 000 -SW columns in series (length 30 cm, internal diameter 0,75 cm) or equivalent columns (e.g. single TSK 2 000 -SWxl, single Agilent Technologies Zorbax GF 250) and a precolumn (3 cm × 0,3 cm) packed with I 125 or material of equivalent effectiveness

4. 6.11.4.

   Thermostatic column oven, set at 35 ± 1 °C

5. 6.11.5.

   Variable wavelength UV detector, permitting measurements at 205 nm with a sensitivity of 0,008 Å

6. 6.11.6.

   Integrator capable of valley-to-valley integration

   Note: Working with columns kept at room temperature is possible, but their power of resolution is slightly lower. In that case, the temperature should vary by less than ± 5 °C in any one range of analyses.