Division 4.1.2 Gel solutionF1

Prepare a gel solution by mixing additives and ampholytes (*) with the stock gel solution (see 4.1.1).

• 9,0 ml stock solution

• 24 mg β-alanine

• 500 μl ampholyte pH 3,5-9,5

• 250 μl ampholyte pH 5-7

• 250 μl ampholyte pH 6-8

Mix the gel solution and de-gas for two to three minutes in an ultrasonic bath or in vacuum.