Section 5 Infection with Aujeszky’s disease virus (ADV)

United Kingdom

	Methods:	Matrix:
ELISA for the detection of antibodies against whole ADV, ADV-gB protein or ADV-gD protein. For batch control of ADV-gB kits and ADV-gD kits or whole ADV kits, Community reference serum ADV 1, or sub-standards, must be scored positive at the dilution of 1:2. When referred to tests for the detection of whole ADV in Part V of Annex IV either of these tests may be used.
ELISA for the detection of antibodies against ADV-gE protein. For batch control, Community reference serum ADV 1, or sub-standards, must be scored positive at the dilution 1:8.
non-vaccinated porcine animals	ADV ELISA^f1	individual or up to 5 pooled serum (or plasma) samples
		individual or up to 5 pooled filter paper samples
		individual meat juice samples
DIVA vaccinated porcine animals with a gE-deleted vaccine	gE ELISA^f2	individual serum samples

Annotations

f1: ELISA for the detection of antibodies against whole ADV, ADV-gB protein or ADV-gD protein. For batch control of ADV-gB kits and ADV-gD kits or whole ADV kits, Community reference serum ADV 1, or sub-standards, must be scored positive at the dilution of 1:2. When referred to tests for the detection of whole ADV in Part V of Annex IV either of these tests may be used.
f2: ELISA for the detection of antibodies against ADV-gE protein. For batch control, Community reference serum ADV 1, or sub-standards, must be scored positive at the dilution 1:8.