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Paragraph 3 Reagents

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3.1

Toluene.

3.2

Sodium sulphate, anhydrous.

3.3

Buffer solution pH1: dissolve 27.2 g sodium acetate trihydrate in 300 ml IM hydrochloric acid and add 700 ml water.

3.4

Buffer solution pH 0.65: dissolve 27.2 g sodium acetate trihydrate in 400 ml IM hydrochloric acid and add 600 ml water.

3.5

Isobutylidenediurea.

3.6

internal standard solution: dilute 5 ml isopropyl acetate to 100 ml with toluene (3.1).