Paragraph 4 apparatus
4.1
250 ml conical flasks with ground glass or PTFE stoppers.
4.2
Stoppered centrifuge tubes.
4.3
Gas chromatograph with flame ionisation detector.
4.4
Column:
-
either (i) 1.5 m glass column (4 mm internal diameter) packed with 5% QV17 on Gas Chrom Q, 80–100 mesh,
-
or (ii) 1.5 m glass column (4 mm internal diameter) packed with 5% Carbowax 20M-TPA on Diatomite C-AAW, 80–100 mesh.
4.5
Water bath: hotplate stirrer on which is placed a 2,000 ml beaker (or suitable vessel) containing water maintained at 40–50°C.