Schedule 4 ANALYTICAL METHODOLOGYI1

Northern Ireland

TABLE A1.

PARAMETERS FOR WHICH, SUBJECT TO REGULATION 16(7), METHODS OF ANALYSIS ARE PRESCRIBED

(1)	(2)
Parameter	Method
Clostridium perfringens (including spores)	Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar* at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.
Coliform bacteria	ISO 9308-1
Colony count 22°C–enumeration of culturable microorganisms	PrEN ISO 6222
Colony count 37°C–enumeration of culturable microoganisms	prEN ISO 6222
Enterococci	ISO 7899-2
Escherichia coli (E. coli)	ISO 9308-1
*The composition of m-CP agar is:
Basal medium
Tryptose	30.0g
Yeast extract	20.0g
Sucrose	5.0g
L-cysteine hydrochloride	1.0g
MgSO₄.7H₂O	0.1g
Bromocresol purple	40.0mg
Agar	15.0g
Water	1,000.0ml
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:
D-cycloserine	400.0mg
Polymyxine-B sulphate	25.0mg
Indoxyl-[beta]-D-glucoside	60.0mg
to be dissolved in 8ml sterile water before addition
Filter-sterilised 0.5% phenolphthalein diphosphate solution	20.0ml
Filter-sterilised 4.5% FeCl₃.6H₂O	2.0ml

TABLE A2.

PARAMETERS IN RELATION TO WHICH METHODS OF ANALYSIS MUST SATISFY PRESCRIBED CHARACTERISTICS

(1)	(2)	(3)	(4)
Parameters	Trueness % of prescribed concentration or value or specification	Precision % of prescribed concentration or value or specification	Limit of detection % of prescribed concentration or value or specification
Notes:
(i) The method of analysis should determine total cyanide in all forms.
(ii) The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned.
(iii) The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1.
(iv) The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1.
(v) The performance characteristics apply to the prescribed value of 4 NTU.
(vi) The performance characteristics apply to the specification of 1 NTU for water leaving treatment works.
Aluminium	10	10	10
Ammonium	10	10	10
Antimony	25	25	25
Arsenic	10	10	10
Benzene	25	25	25
Benzo(a)pyrene	25	25	25
Boron	10	10	10
Bromate	25	25	25
Cadmium	10	10	10
Chloride	10	10	10
Chromium	10	10	10
Colour	10	10	10
Conductivity	10	10	10
Copper	10	10	10
Cyanide ^f1	10	10	10
1,2-dichloroethane	25	25	10
Fluoride	10	10	10
Iron	10	10	10
Lead	10	10	10
Manganese	10	10	10
Mercury	20	10	20
Nickel	10	10	10
Nitrate	10	10	10
Nitrite	10	10	10
Pesticides and related products ^f2	25	25	25
Polycyclic aromatic hydrocarbons ^f3	25	25	25
Selenium	10	10	10
Sodium	10	10	10
Sulphate	10	10	10
Tetrachloroethene ^f4	25	25	10
Tetrachloromethane	20	20	20
Trichloroethene	25	25	10
Trihalomethanes: Total	25	25	10
Turbidity ^f5	10	10	10
Turbidity ^f6	25	25	25

Annotations

f1

(i)

The method of analysis should determine total cyanide in all forms.

f2

(ii)

The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned.

f3

(iii)

The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1.

f4

(iv)

The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1.

f5

(v)

The performance characteristics apply to the prescribed value of 4 NTU.

f6

(vi)

The performance characteristics apply to the specification of 1 NTU for water leaving treatment works.