Schedule 3 ANALYSIS: METHODS AND CAPABILITIES
TABLE M1
SPECIFIED PARAMETERS: ANALYTICAL METHOD TO BE USED
(1) Item | (2) Parameter | (3) Method | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
(a) The composition of m-CP agar is: Basal medium— Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes.
Allow the medium to cool and add—
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| 1. | Clostridium perfringens (including spores) | Membrane filtration followed by anaerobic incubation of the membrane on m-CP agarf1 at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds. | ||||||||||||||||||||||||||
| 2. | Coliform bacteria | ISO 9308-1 | ||||||||||||||||||||||||||
| 3. | Colony count 22°C — enumeration of culturable microorganisms | prEN ISO 6222 | ||||||||||||||||||||||||||
| 4. | Colony count 37°C — enumeration of culturable microorganisms | prEN 6222 | ||||||||||||||||||||||||||
| 5. | Enterococci | ISO 7899-2 | ||||||||||||||||||||||||||
| 6. | Escherichia coli | ISO 9308-1 | ||||||||||||||||||||||||||
| Notes— | ||||||||||||||||||||||||||||
TABLE M2
OTHER PARAMETERS: CAPABILITIES REQUIRED OF ANALYTICAL METHOD
| (1) Item | (2) Parameter | (3) Trueness % of prescribed concentration or value | (4) Precision % of prescribed concentration or value | (5) Limit of detection % of prescribed concentration or value |
|---|---|---|---|---|
| Notes— | ||||
(a) The method of analysis should determine total cyanide in all forms. | ||||
(b) The capabilities required apply in relation to each individual pesticide. | ||||
(c) The capabilities required apply in relation to each individual substance comprising PAH: Total at 25% of the prescribed concentration or value for PAH: Total. | ||||
(d) The capabilities required apply at 50% of the prescribed concentration or value for PAH: Total. | ||||
(e) The capabilities required apply in relation to prescribed concentration or value in Table B. | ||||
(f) The capabilities required apply in relation to prescribed concentration or value in Table C. | ||||
| In this Schedule “Pesticide”, “PAH: Total” and “THM: Total” have the meanings given in Schedule 1. | ||||
| 1. | Aluminium | 10 | 10 | 10 |
| 2. | Ammonium | 10 | 10 | 10 |
| 3. | Antimony | 25 | 25 | 25 |
| 4. | Arsenic | 10 | 10 | 10 |
| 5. | Benzene | 25 | 25 | 25 |
| 6. | Benzo(a)pyrene | 25 | 25 | 25 |
| 7. | Boron | 10 | 10 | 10 |
| 8. | Bromate | 25 | 25 | 25 |
| 9. | Cadmium | 10 | 10 | 10 |
| 10. | Chloride | 10 | 10 | 10 |
| 11. | Chromium | 10 | 10 | 10 |
| 12. | Colour | 10 | 10 | 10 |
| 13. | Conductivity | 10 | 10 | 10 |
| 14. | Copper | 10 | 10 | 10 |
| 15. | Cyanidef2 | 10 | 10 | 10 |
| 16. | 1,2-dichloroethane | 25 | 25 | 10 |
| 17. | Fluoride | 10 | 10 | 10 |
| 18. | Iron | 10 | 10 | 10 |
| 19. | Lead | 10 | 10 | 10 |
| 20. | Manganese | 10 | 10 | 10 |
| 21. | Mercury | 20 | 10 | 20 |
| 22. | Nickel | 10 | 10 | 10 |
| 23. | Nitrate | 10 | 10 | 10 |
| 24. | Nitrite | 10 | 10 | 10 |
| 25. | Pesticidef3 | 25 | 25 | 25 |
| 26. | PAH: Totalf4 | 25 | 25 | 25 |
| 27. | Selenium | 10 | 10 | 10 |
| 28. | Sodium | 10 | 10 | 10 |
| 29. | Sulphate | 10 | 10 | 10 |
| 30. | Tetrachloroethenef5 | 25 | 25 | 10 |
| 31. | Tetrachloromethane | 20 | 20 | 20 |
| 32. | Trichloroethene | 25 | 25 | 10 |
| 33. | THM: Total | 25 | 25 | 10 |
| 34. | Turbidityf6 | 10 | 10 | 10 |
| 35. | Turbidityf7 | 25 | 25 | 25 |
Annotations
Footnotes
- f1
(a)
The composition of m-CP agar is:
Basal medium—
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes.
Tryptose 30.0 g Yeast extract 20.0 g Sucrose 5.0 g L-cysteine hydrochloride 1.0 g MgSO4.7H2O 0.1 g Bromocresol purple 40.0 mg Agar 15.0 g Water 1,000.0 ml Allow the medium to cool and add—
D-cycloserine 400.0 mg Polymyxine-B sulphate 25.0 mg Indoxyl-(beta)-D-glucoside to be dissolved in 8ml sterile water before addition 60.0 mg Filter-sterilised 0.5% phenolphthalein disphosphate solution 20.0 ml Filter-sterilised 4.5% FeCl3.6H2O 2.0 ml - f2
(a)
The method of analysis should determine total cyanide in all forms.
- f3
(b)
The capabilities required apply in relation to each individual pesticide.
- f4
(c)
The capabilities required apply in relation to each individual substance comprising PAH: Total at 25% of the prescribed concentration or value for PAH: Total.
- f5
(d)
The capabilities required apply at 50% of the prescribed concentration or value for PAH: Total.
- f6
(e)
The capabilities required apply in relation to prescribed concentration or value in Table B.
- f7
(f)
The capabilities required apply in relation to prescribed concentration or value in Table C.