Division II.1
United Kingdom
Organs to be sampled:
The tissue material to be examined is spleen, anterior kidney, and either heart or encephalon. When sampling broodstock ovarian or seminal fluid may also be examined.
In case of small fry, whole fish less than 4 cm long can be minced with sterile scissors or a scalpel after removal of the body behind the gut opening. If a sample consists of whole fish with a body length between 4 cm and 6 cm, the viscera including kidney shall be collected.
Organ pieces from a maximum of 10 fish may be pooled.
Notes on Part 1
Footnotes
- f1
The samples must be collected no sooner than 3 weeks after the transfer of the fish from fresh to saltwater.
- f2
Ovarian or seminal fluid of broodstock shall be collected at the time of maturation, in connection with stripping.
- f3
Samples must be taken from the number of fish that will ensure the detection of VHSV or IHNV with a 95 % confidence if the design prevalence is 5 %.
- f4
The samples must be collected no sooner than 3 weeks after the transfer of the fish from fresh to saltwater.
- f5
Ovarian or seminal fluid of broodstock shall be collected at the time of maturation, in connection with stripping.
- f6
Samples must be taken from the number of fish that will ensure detection of VHSV or IHNV with a 95 % confidence if the design prevalence is 10 %.
- f7
There shall be minimum one sample for every health inspection.
- f8
The samples must be collected no sooner than 3 weeks after the transfer of the fish from fresh to saltwater.
- f9
Samples must be taken from the number of fish that will ensure the detection of VHSV or IHNV with a 95 % confidence if the design prevalence is 10 %.