Division II.2 Diagnostic methods to obtain and maintain disease-free status for either VHS or IHN or both

United Kingdom

The diagnostic method, in accordance with the approved diagnostic methods and procedures set out in point I. of Part 1 of Annex II, to achieve or to maintain disease-free status for VHS or IHN or both shall be either:

(a)
virus isolation in cell cultures followed by identification using enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT), virus neutralisation test or real-time reverse transcriptase polymerase chain reaction (RT-qPCR); or
(b)
RT-qPCR.

Notes on Part 1

Footnotes

f1: The samples must be collected no sooner than 3 weeks after the transfer of the fish from fresh to saltwater.
f2: Ovarian or seminal fluid of broodstock shall be collected at the time of maturation, in connection with stripping.
f3: Samples must be taken from the number of fish that will ensure the detection of VHSV or IHNV with a 95 % confidence if the design prevalence is 5 %.
f4: The samples must be collected no sooner than 3 weeks after the transfer of the fish from fresh to saltwater.
f5: Ovarian or seminal fluid of broodstock shall be collected at the time of maturation, in connection with stripping.
f6: Samples must be taken from the number of fish that will ensure detection of VHSV or IHNV with a 95 % confidence if the design prevalence is 10 %.
f7: There shall be minimum one sample for every health inspection.
f8: The samples must be collected no sooner than 3 weeks after the transfer of the fish from fresh to saltwater.
f9: Samples must be taken from the number of fish that will ensure the detection of VHSV or IHNV with a 95 % confidence if the design prevalence is 10 %.