OpenLegislation

Division 4 Additional instructions:F1F2

United Kingdom

Original text. Later amendments are not shown.

  1. (a)

    the experiment shall be carried out with two serum dilutions (1:5 and 1:10) in order to achieve optimal precipitation;

  2. (b)

    if the precipitation diameter is too small with both dilutions, then the serum shall be further diluted;

  3. (c)

    if the precipitation diameter in both dilutions is too large and faint, then a lower serum shall be chosen;

  4. (d)

    the final concentration of the agarose shall be 0,8 %; that of the sera 5 and 10 % respectively;

  5. (e)

    plot the measured diameters in the following coordinate system. The dilution of the antigen to be tested with the same diameter as the reference antigen is the working dilution.

Annotations

Textual amendments

F1

Substituted by Council Directive 97/12/EC of 17 March 1997 amending and updating Directive 64/432/EEC on health problems affecting intra-Community trade in bovine animals and swine.

F2

Substituted by Commission Decision of 15 December 2009 amending Annex D to Council Directive 64/432/EEC as regards diagnostic tests for enzootic bovine leukosis (notified under document C(2009) 9951) (Text with EEA relevance) (2009/976/EU).

Notes on Annex D

Textual amendments

F2

Substituted by Council Directive 98/46/EC of 24 June 1998 amending Annexes A, D (Chapter I) and F to Directive 64/432/EEC on health problems affecting intra-Community trade in bovine animals and swine.