Division 3 PRINCIPLE

The reconstituted sample is diluted with a buffer substrate at pH 10,2 and incubated at a temperature of 37 ^oC for two hours. Any alkaline phosphatase present in the sample will, under these circumstances, liberate p-nitrophenol from added disodium p-nitrophenyl phosphate. The p-nitrophenol liberated is determined by direct comparison with standard colour glasses in a simple comparator using reflected light.